A library of expression clones and cellular manipulation tools

We have generated an extensive library of C. elegans promoters as Invitrogen Gateway entry clones to facilitate easy cloning of tissue-specific reporters. As a lab that focuses on males, we have analyzed the expression of many of the promoters in the male. In addition to an extensive list of published promoters (Table 1) (Gruninger et al., 2006; Gruninger et al., 2008; LeBoeuf et al., 2007; Liu et al., 2007; Reiner et al., 2006), we have a large library of promoters in entry clones that have not been published (Table 2), and we wish to make these plasmids available to the worm community. We have also included a list of Gateway destination vectors we have generated (Table 3). Plasmids available are listed in the tables below.

Contact Dr. Rene Garcia to obtain plasmids of interest.


Table 1
Figure 1
Table 2
Figure 2
Table 3
Figure 3


Gruninger TR, Gualberto DG, LeBoeuf B, and Garcia LR. (2006). Integration of male mating and feeding behaviors in Caenorhabditis elegans. J. Neurosci. 26, 169-179. PubMed

Gruninger TR, Gualberto DG, and Garcia LR. (2008). Sensory perception of food and insulin-like signals influence seizure susceptibility. PLoS Genet 4:e1000117. PubMed

LeBoeuf B, Gruninger TR, and Garcia LR. (2007). Food deprivation attenuates seizures through CaMKII and EAG K+ channels. PLoS Genetics 3:e156. PubMed

Liu Y, LeBoeuf B, and Garcia LR. (2007). G alpha(q)-coupled muscarinic acetylcholine receptors enhance nicotinic acetylcholine receptor signaling in Caenorhabditis elegans mating behavior. J. Neurosci. 27, 1411-1421. PubMed

Reiner DJ, Weinshenker D, Tian H, Thomas JH, Nishiwaki K, Miwa J, Gruninger T, LeBoeuf B, and Garcia LR. (2006). Behavioral genetics of Caenorhabditis elegans unc-103-encoded erg-like K(+) channel. J. Neurogenet. 20, 41-66. PubMed

Published: December 6, 2010 in

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