C. elegans CRISPR/Cas papers

Scalable and versatile genome editing using linear DNAs with micro-homology to Cas9 Sites in Caenorhabditis elegans.  Paix A, Wang Y, Smith H, Lee CY, Calidas D, Lu T, Smith J, Schmidt H, Krause M, and Seydoux G. Genetics (2014). Sep 23. pii: genetics.114.170423. [Epub ahead of print] PMID: 25249454

Efficient marker-free recovery of custom genetic modifications with CRISPR/Cas9 in Caenorhabditis elegans. Arribere JA, Bell RT, Fu BX, Artiles KL, Hartman PS, and Fire AZ. Genetics (2014). Aug 26. pii: genetics.114.169730. [Epub ahead of print] PMID: 25161212

Conditional knockouts generated by engineered CRISPR-Cas9 endonuclease reveal the roles of Coronin in C. elegans neural development. Shen Z, Zhang X, Chai Y, Zhu Z, Yi P, Feng G, Li W, and Ou G. Dev. Cell (2014). 30, 625-636. Epub 2014 Aug 21. PMID: 25155554

Efficient site-specific editing of the C. elegans genome. Zhang D, and Glotzer M. bioRxiv (2013). July 21. http://dx.doi.org/10.1101/007344

A Co-CRISPR strategy for efficient genome editing in Caenorhabditis elegans. Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, and Mello CC. Genetics (2014). 197, 1069-1080. PMID: 24879462

Heritable/conditional genome editing in C. elegans using a CRISPR-Cas9 feeding system. Liu P, Long L, Xiong K, Yu B, Chang N, Xiong JW, Zhu Z, and Liu D. Cell Res. (2014). 24, 886-889. doi: 10.1038/cr.2014.73. PMID: 24874953

Engineering the Caenorhabditis elegans genome with CRISPR/Cas9. Waaijers S, and Boxem M. Methods (2014). 68, 381-388. doi: 10.1016/j.ymeth.2014.03.024. PMID: 24685391

Oligonucleotide-based targeted gene editing in C. elegans via the CRISPR/Cas9 system. Zhao P, Zhang Z, Ke H, Yue Y, and Xue D. Cell Res. (2014). 24, 247-250. doi: 10.1038/cr.2014.9. PMID: 24418757

Versatile strategy for isolating transcription activator-like effector nuclease-mediated knockout mutants in Caenorhabditis elegans. Sugi T, Sakuma T, Ohtani Y, and Yamamoto T. Dev. Growth Differ. (2014). 56, 78-85. doi: 10.1111/dgd.12108. PMID: 24409999

Using the CRISPR/Cas9 system to target cis-regulatory sequences in C. elegans. Michalis Barkoulas, Aurelien Richaud and Marie-Anne Félix. WBG 20 #1, March 2014

Exciting prospects for precise engineering of Caenorhabditis elegans genomes with CRISPR/Cas9. Frøkjær-Jensen C. Genetics (2013). 195, 635-642. doi: 10.1534/genetics.113.156521. PMID: 24190921

Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination. Chen C, Fenk LA, and de Bono M. Nucleic Acids Res. (2013). 41, e193. doi: 10.1093/nar/gkt805. PMID: 24013562

Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Dickinson DJ, Ward JD, Reiner DJ, and Goldstein B. Nat. Methods (2013). 10, 1028-1034. doi: 10.1038/nmeth.2641. PMID: 23995389

CRISPR/Cas9-targeted mutagenesis in Caenorhabditis elegans. Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den Heuvel S, and Boxem M. Genetics (2013). 195, 1187-1191. doi: 10.1534/genetics.113.156299. PMID: 23979586

Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system. Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, and Colaiácovo MP. Genetics (2013). 195, 1181-115. doi: 10.1534/genetics.113.156075. PMID: 23979579

Targeted heritable mutation and gene conversion by Cas9-CRISPR in Caenorhabditis elegans. Katic I, and Großhans H. Genetics (2013). 195, 1173-116. doi: 10.1534/genetics.113.155754. PMID: 23979578

Transgene-free genome editing in Caenorhabditis elegans using CRISPR-Cas. Chiu H, Schwartz HT, Antoshechkin I, and Sternberg PW. Genetics (2013). 195, 1167-1171. doi: 10.1534/genetics.113.155879. PMID: 23979577

Heritable gene knockout in Caenorhabditis elegans by direct injection of Cas9-sgRNA ribonucleoproteins. Cho SW, Lee J, Carroll D, Kim JS, and Lee J. Genetics (2013). 195, 1177-1180. doi: 10.1534/genetics.113.155853. PMID: 23979576

Precise and heritable genome editing in evolutionarily diverse nematodes using TALENs and CRISPR/Cas9 to engineer insertions and deletions. Lo TW, Pickle CS, Lin S, Ralston EJ, Gurling M, Schartner CM, Bian Q, Doudna JA, and Meyer BJ. Genetics (2013). 195, 331-348. doi: 10.1534/genetics.113.155382. PMID: 23934893

Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Friedland AE, Tzur YB, Esvelt KM, Colaiácovo MP, Church GM, and Calarco JA. Nat. Methods (2013). 10, 741-743. doi: 10.1038/nmeth.2532. PMID: 23817069

 

Published: October 20, 2014 in