CGC Bibliography Paper 4883
Genetic analysis of endocytosis in Caenorhabditis elegans: coelomocyte uptake defective mutants.
Fares H,
Greenwald I
- Medline:
- 11560892
- Citation:
- Genetics 159: 133-145 2001
- Type:
- ARTICLE
- Genes:
- bli-5 ced-2 ced-5 ced-9 cha-1 cup-1 cup-2 cup-3 cup-4 cup-5 cup-6 cup-7 cup-8 cup-9 cup-10 cup-11 cyk-1 dpy-5 dpy-7 dpy-9 dpy-11 dpy-13 dpy-14 dpy-17 dpy-18 dpy-24 dyf-2 dyf-3 dyf-4 dyf-5 dyf-13 dyn-1 eat-1 eat-7 eat-10 eat-20 emb-29 glp-4 him-5 lag-2 lin-1 lin-10 mab-18 mig-2 nrf-4 nrf-5 nrf-6 pod-1 rme-1 rme-6 rme-8 rol-6 sec-5 sec-8 sma-1 sma-3 snt-1 sqt-3 unc-4 unc-6 unc-11 unc-13 unc-17 unc-18 unc-25 unc-26 unc-29 unc-30 unc-31 unc-32 unc-33 unc-34 unc-36 unc-39 unc-42 unc-54 unc-60 unc-64 unc-69 unc-73 unc-76 unc-78 unc-97 unc-101 unc-104 unc-115 sDf26 sDf28 sDf31 sDf32 sDf42 sDf45 sDf46 sDf60
- Abstract:
- The coelomocytes of Caenorhabditis elegans are scavenger cells that continuously and nonspecifically endocytose fluid front the pseudococlom (body cavity). Green fluorescent protein (GFP) secreted into the pseudocoelom from body wall muscle cells is endocytosed and degraded by coelomocytes. We show that toxin-mediated ablation of coelomocytes results in viable animals that fail to endocytose pseudocoelomic GFP, indicating that endocytosis by coelomocytes is not essential for growth or survival of C. elegans tinder normal laboratory, conditions. We examined known viable endocytosis mutants, and performed RNAi for other known endocytosis genes, for coelomocyte uptake defective (Cup) phenotyes. We also screened fur riew genes involved in endocytosis by isolating viable mutants with Cup defects; this screen identified 14 different genes, many with multiple alleles. A variety of Cup terminal phenotypes were observed, consistent with defects at various steps in the endocytic pathway. Available molecular information indicates that the Cup mutant screen has identified novel components of the endocytosis machinery that are conserved in mammals but not in Saccharomyces cerevisiae, the only other organism for which largescale genetic screens for endocytosis mutants have