CGC Bibliography Paper 4963

DAF-7/TGF-B expression required for the normal larval development in C. elegans is controlled by a presumed guanylyl cyclase DAF-11.

Murakami M, Koga M, Ohshima Y

Medline:

11677050

Citation:

Mechanisms of Development 109: 27-35 2001

Type:

ARTICLE

Genes:

che-2 che-3 daf-1 daf-2 daf-3 daf-4 daf-5 daf-6 daf-7 daf-8 daf-10 daf-11 daf-12 daf-14 daf-16 daf-21 daf-22 gpa-4 gpa-9 gpa-10 gpa-14 lin-44

Abstract:

In C. elegans development, unfavorable growth conditions lead a larva to an arrested and enduring form called a dauer. To elucidate components upstream of DAF-7/TGF-beta in this control pathway, we isolated a mutant that was defective in daf-7 promoter::gfp reporter expression and showed an arrested (dauer-constitutive) phenotype. It has a new mutation in the daf-11 gene encoding a transmembrane guanylyl cyclase. We show that daf-11 gene and a related gene daf-21 act upstream of daf-7, and cilium-related genes che-2 and che-3 are placed between daf-11 and daf-7, in the genetic pathway controlling dauer formation. Expression of daf-11 cDNA by cell specific promoters suggests that daf-11 acts cell autonomously in ASI chemosensory neurons for daf-7 expression.