CGC Bibliography Paper 5097

Identification of a dopamine receptor from Caenorhabditis elegans.

Suo S, Sasagawa N, Ishiura S

Medline:

11814642

Citation:

Neuroscience Letters 319: 13-16 2002

Type:

ARTICLE

Genes:

Abstract:

The neurotransmitter dopamine regulates locomotion and egg laying in the nematode Caenorhabditis elegans. We have cloned a cDNA encoding the C. elegans G protein-coupled receptor (CeDOP1). The deduced amino acid sequence of the cloned cDNA shows high sequence similarities with D1-like dopamine receptors from other species. Three splice variants that differ in the length of the predicted third intracellular loop and C-terminal tail were identified. COS-7 cells transiently transfected with CeDOP1 showed high affinity binding to [1251] iodo-lysergic acid diethylamide (K-D = 3.43 +/- 0.83 nM). Dopamine showed the highest affinity (K-i = 0.186 muM) for this receptor among several vertebrate and invertebrate amine neurotransmitters tested, suggesting that the natural ligand for this receptor is dopamine.