CGC Bibliography Paper 5108

Cytoskeletal regulation by the Nedd8 ubiquitin-like protein modification pathway.

Kurz T, Pintard L, Willis JH, Hamill DR, Gonczy P, Peter M, Bowerman B

Medline:

11847342

Citation:

Science 295: 1294-1298 2002

Type:

ARTICLE

Genes:

air-2 cyk-1 cul-2 cul-3 mei-1 mei-2 mlc-4 ned-8 rfl-1 ubc-12 ula-1 zen-4

Abstract:

The Nedd8 ubiquitin-like protein modification pathway regulates cell-cycle progression. Our analysis of Nedd8 requirements during Caenorhabditis elegans embryogenesis indicates that the cytoskeleton is another target. Nedd8 conjugation negatively regulated contractility of the microfilament-rich cell cortex during pronuclear migration and again during cytokinesis. The Nedd8 pathway also was required after meiosis to negatively regulate katanin, a microtubule-severing complex, permitting the assembly of a large mitotic spindle. We propose that Nedd8-modified cullin, as part of an E3 ubiquitin ligase complex, targets katanin for degradation during the transition from meiosis to mitosis.