Worm Breeder's Gazette 11(2): 56
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Although the unc-104 fragments isolated from an Arhinger-Kimble library were instrumental in obtaining approximately 4 kb of the 6 kb mRNA sequence, it was desirable to obtain full length cDNA clones to both complete the cDNA sequence and to produce the recombinant protein product in bacteria. The discrepancy between the abundance of unc-104 cDNA clones in the Arhinger-Kimble library (approximately 1 per 10,000) and in the Kim-Horvitz (none in 200,000) and Thacker-Capecci libraries (none in 100,000), led us to screen the Barstead-Waterston and Schauer-Wood libraries. The abundance of cDNA clones in these additional libraries suggests that an insufficient number of clones was screened in the cases of the Kim and Thacker libraries. cDNA clones were isolated from 2.6 million phage (1.4 million phage screened from the Barstead-Waterston bank, 0.6 million the Schauer- Wood early embryonic and him-8 embryonic libraries). The probe was the 5'-most cDNA fragment obtained from the library. The results are shown below: [See Figure 1] The two cDNA clones from the Barstead library overlap and cover a region of 4.7 kb. DNA sequencing of the cDNA clones is currently in progress.