Worm Breeder's Gazette 11(2): 60
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
From a Tc1 mutagenesis screen in the nT1(IV,V) balanced region using mut-4 RW7037, six lethal mutations that fail to complement lin-40 were recovered (Clark et al. In Press Genome). We have extracted DNA from the six strains containing lin-40 Tc1 mutations. We digested the DNA with EcoRI, ran it on a gel, transfered the DNA to a filter and hybridized nick translated Tc1 DNA to it. Three strains showed a common new Tc1 band. For one allele (s1351) we recovered the new band, isolated DNA flanking the Tc1 sequence and used the flanking sequence to screen T. Snutch's Charon IV library. From the library we isolated a phage (BC#S1001). DNA from BC#S1001 was mapped to LGV(left) on the cosmid map by J. Sulston and A. Coulson (see figure). The location is surprising in that BC#S1001 maps very close to unc-60 molecularly (unc-60 is in F53E2 pers. comm. M. Wakarchuk) but genetically lin-40 is fairly far away (roughly 9 m.u.) to the right of unc-60. We are attempting to rescue lin-40 by microinjection using appropriate cosmids. We are also screening for revertants of some of the Tc1 alleles of lin-40. This research has been supported by an MRC studentship to RCJ and NSERC and MDA (Canada) grants to DLB. [See Figure 1]