Worm Breeder's Gazette 12(1): 19 (September 1, 1991)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Transposon Tagging of unc-51 Gene

Ken-ichi Ogura, Ikue Mori, Yasumi Ohshima

Department of Biology, Faculty of Science, Kyushu University, Higashi-ku, Fukuoka 812, Japan

In unc-51 mutants, several neurons have abnormally guided axons (HSN, C. Desai et al. Nature. 336 (1988) 638-646; PDE, amphid and phasmid neurons, E. Hedgecock et al. Dev. Biol. 111 (1985) 158-170 etc.). Several unc mutants were found among progenies of mutator strain RW7097 .One of them did not complement unc-51 ( e369 ),and it was tentatively named as UF. UF reverted at a high frequency. These results suggested that UF carried Tc1 insertion in the unc-51 gene. To analyze the function of unc-51 gene, we tried to clone this gene with Tc1 probe. To reduce the number of Tc1 copies, UF was crossed with N2 ten times. This strain was named unc-51 ( ks38 ::T c1 ).Southern blot analysis showed that the unc-51 ( ks38 ::T c1 )contained about ten extra Tc1s when compared with N2 .To find a Tc1 which had inserted into the unc-51 gene, RFLP analysis was done with flanking DNA of extra Tc1s .One clone hybridized with a 4.3kb EcoRI fragment in unc-51 ( ks38 ::T c1 ),but with a 2.7kb EcoRI fragment in revertants and N2 .This DNA fragment may contain a part of unc-51 gene. To map the DNA fragment, we are sending this clone to MRC. If this clone is mapped near the unc-51 gene, we will try to clone cDNA and to analyze the genomic and cDNA clones.