Worm Breeder's Gazette 12(1): 39 (September 1, 1991)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Complete sequence of tropomyosin gene, tmy-1 ,LGV of Caenorhabditis elegans

Hirosi Matsumoto, Yasuji Sakube, Koken Sugimoto, Hiroaki Kagawa

Figure 1

Department of Biology, Faculty of Science, Okayama University, Okayama 700, JAPAN (C51918@JPNKUDPC)

Tropomyosin is a thin filament component of muscle structure. We cloned and sequenced genomic DNA and three species of cDNA. Tmy-1 gene encoded 16 exons and was expanding more than 12kb. All of this sequence located between cosmid clones F39B2 and K05C4 (just left of unc-54 I, no overlap cosmid and phage clones). The tmy-1 gene alternatively spliced into 3 isoforms. Two muscle and one cytoplasmic types were similar splicing patterns to that of other animals (Drosophila TmII gene and chicken tropomyosin gene). The part of exon1 and exon2 was matched to the result of PCR experiment (Larry Schiliefer and Bob Waterston, personal communication). Two muscle types had identical amino acid sequences except 27 amino acid residues of C-terminal end. There was a typical promoter like sequence in 5' non-coding region of two muscle types (Fig.1). 5' end of the last cDNA was still missing. The same poly A signal was used in three isoforms, although there were other poly A signals in the 3' downstream of the gene.

Tmy-1 gene expression of transcription factor control and splicing mechanism of tissue specific isoform are interested.

[See Figure 1]

Figure 1