Worm Breeder's Gazette 14(1): 104 (October 1, 1995)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
box 45, Novosibirsk 630107, USSR
Three adult animals were kept in microtitre wells containing 0,75 ml of liquid medium with E. coli during 12 hours, then they were discarded and newborn larvae were transferred in next wells every day (one worm in one well). After second day number of progeny was calculated, if any animal during 2 days stopped reproduction, I used new medium with ethanol in different concentrations: 0,0% (control), 0,5%, 0% and 2,0%, 12 nematodes with every ethanol concentration. Ethanol concentration 0,0% 0,5% 1,0% 2,0% Mean life span (days) 23,6+/-1,8 20,1+/-1,0 19,5+/-0,9 22,0+/-1,4 Mean life span after ethanol addition (days) - 5,4+/-0,9 3,8+/-0,6 5,2+/-1,5 Number of progeny from 85,7+/-7,9 85,2+/-6,4 92,5+/-10,2 103,3+/-12,3 single worm Maximal number of progeny from one worm after - 35 44 60 ethanol addition It can be concluded, that ethanol has no effect on life span of aged C. elegans, but the fecundity slightly increased with increasing ethanol concentration. In 7 cases ethanol renewed reproduction in C. elegans after 2 day ceasing , although there was no life prolongation. Acknowledgment: The author wishes to express his gratitude to CGC for providing C. elegans (Bristol, N2, wild line) and E. coli OP50.