Worm Breeder's Gazette 15(4): 35 (October 1, 1998)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Null Mutations in tig-2, a TGFb-like Ligand

Huang Wang, Lisa Maduzia, Srik Krishna, Richard W. Padgett

Waksman Institute, Rutgers University, Piscataway, NJ 08854-8020

Three TGFb-like pathways have been defined in C. elegans: the 
Sma/Mab pathway that responds to dbl-1, the dauer pathway that 
responds to daf-7, and the unc-129 pathway. We have been 
examining tig-2, which is a TGFb-like ligand most similar to 
the BMP5-7/60A class of ligands. If the functions of tig-2 
parallel those of its Drosophila counterpart, then it will 
partially function together with the BMP-like homologs, 
dbl-1 or daf-7. To address this issue and to determine its 
mutant phenotype, we have generated null mutations in tig-2.
We opted to isolate deletions using a PCR-based screen that 
closely parallels the method developed by B. Barstead and 
G. Moulder (http://snmc01.omrf.ouhsc.edu/revgen/RevGen.html).
In support of this approach, the nematode groups at Rutgers 
have coordinated the construction of several "deletion" 
libraries, and have successfully obtained mutants in several 
genes. Using this method, we have isolated a 1.5 kb deletion
in tig-2, which removes most of the coding sequences. We 
have observed that homozygous animals are viable and the 
analysis of their mutant phenotype is underway. Further 
studies will determine if tig-2 defines a separate pathway
or functions in one of the three known TGFb-like 
pathways of C. elegans.