Worm Breeder's Gazette 15(4): 46 (October 1, 1998)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

An Attempt to Slow Aging in C. elegans. 10. The Slight Positive Effect of C. elegans Larvae Extract

Vladimir Bakaev, Andrey P. Ivlev-Duntau

Box 45, Novosibirsk, 630107 Russia

C. elegans larvae (1-2 days old) were collected from separate stock,
frozen and after 12 hours or more time, thawed, centrifuged and mixed
with S medium containing E. coli (1:1), this mixture was used as a
medium for worms in experimental group.  The medium for the control
group was prepared by mixing S medium containing E. coli with S medium
without E. coli (1:1).  Three adult animals (3-5 days old) were kept in
microtitre wells containing 0.5 ml of liquid medium (with E. coli and
with or without larvae extract) during 12 hours, then they were
transferred in next wells everyday (one worm in one well).  Dilutions of
larvae extract (1:10, 1:100 and 1:1000) were applied on third day.  This
investigation was carried out in temperature +24C and in the darkness. 
The obtained results are presented in the following table.

Dilutions of                              Longevity (days)
larvae extract          n          mean +/- S.E.          maximal
No dilution            12                         toxic                  
1:10                   12          19.9 +/- 1.5             25
1:100                  12          15.0 +/- 1.4             27
1:1000                 12          14.6 +/- 1.5             22
Control                12          16.0 +/- 1.6             20

Conclusion: If the larvae extract was applied to C. elegans, it was able
slightly to increase their mean longevity (by 24.4 percent, not
significant) in dilution 1:10.  Probably extract prepared from larvae C.
elegans contains any factor, which can slightly stimulate longevity of
adult worms, but this effect is obviously insufficient for noticeable
prolonging of longevity.

Acknowledgment: The authors wish to express his thanks to CGC for
providing C. elegans(Bristol, N2) and E. coli OP50.