Worm Breeder's Gazette 5(1): 32b
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
A temperature-dependent morphologically abnormal mutant was isolated, which develops into an adult with dumpy phenotype at 15 C but with roller phenotype at 25 C in addition to dumpy. At 25 C, the dumpy phenotype appeared within L1 stage and was complete at L3 stage and the roller phenotype was also appeared within L3 stage. The heterozygous offspring( F1 ), produced by crossing the mutant hermaphrodite with wild-type males, were rollers. Therefore, the dumpy is autosomal recessive and the roller is dominant in heterozygote but temperature-sensitive in homozygote. The F2 progeny produced by self-fertilization of the F1 roller hermaphrodite segregated to wild, dumpy, and roller animals in a ratio of 1:1:2. The dumpy mutation in the mutant was shown to be an allele of dpy-10 ( LG II ). To segregate the roller mutation from the dumpy, 103 F2 roller individuals were cloned, but no homozygous roller progeny was obtained. Therefore, the dumpy and the roller phenotypes are produced by closely linked mutations or perhaps by a single mutation. In the mutant, the epistatic effect of the dumpy to roller may be temperature- sensitive.