Worm Breeder's Gazette 7(1): 50
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Six dumpy mutants were contributed to the CGC collection by the Lyon laboratory. Three strains were reported by Roland Ouazana to carry X- linked mutations, two carried autosomal mutations, and one apparently had not been tested (FF17). Each strain was crossed with appropriate Bristol males for mapping and complementation, and the results are summarized as follows: [See Figure 1] FF1 maps to LG I, complements dpy-14(e188), but is allelic with dpy- 5(e61). FF14 is said to be representative of a frequently mutated gene, and it is allelic with dpy-1(e1). The remaining four dumpy mutants are all X-linked, so they first were tested for complementation with ts alleles of dpy-3(m39), 24), and dpy-8(e1281). All the mutants complemented these alleles. Since we have no ts alleles of dpy-6, we performed those complementation tests using tra-1(e1099)II males to construct the Bergerac/Bristol hybrids. The tra-1/tra-1; re crossed with Bergerac dumpy mutants at 25 C to select against selfed progeny. Dumpy hermaphrodite progeny subsequently were cloned to check segregation of the paternal tra-1 marker. All four X-linked strains are allelic with dpy-6(e14).At this point, we see no reason to invoke special rules of nomenclature for Bergerac alleles. However, any strain or allele generated in a Bergerac background should be clearly designated as such in its description, along with mutagen used, temperature-sensitivity, and other important genetic and phenotypic descriptors.