Table of Contents
Nuclear receptors are transcription factors that often respond to small molecule metabolites and fat-soluble compounds to regulate gene expression. They broadly govern development, reproduction, metabolism, and homeostasis in diverse metazoan species and their dysregulation is associated with numerous diseases. Work in C. elegans has shed light on the seminal role of nuclear receptors in life history regulation, stem cell progression, developmental timing, cell fate specification, nutrient sensing, metabolism, and longevity. Here we highlight recent advances on the best-studied nuclear receptors in the worm, and how they illuminate metazoan biology.
NR: nuclear receptor; LBD: ligand binding domain, DA: dafachronic acid; IIS: insulin/IGF signaling; AC: anchor cell; ARD: adult reproductive diapause; LC: linker cell; MUFA: monounsaturated fatty acid; PUFA: polyunsaturated fatty acid; TAG: triacyl glycerides; VU: ventral uterine; DR: Dietary restriction.
A remarkable invention of metazoan evolution, nuclear receptors (NR) are ligand gated transcription factors that typically bind small molecule metabolites such as fatty acids, vitamins, and steroids to directly regulate gene transcription. They are well poised to coordinate multicellular metabolism, development, reproduction, and homeostasis across diverse tissues. A conserved architecture and mechanism underlies their signaling abilities (Mangelsdorf et al., 1995). The DNA binding domain consists of two zinc fingers near the N-terminus, which contact the double helix and form a dimerization interface. The ligand binding domain (LBD) resides at the C-terminus, and binds cognate ligand as well as co-activator and co-repressor complexes that instruct its activity. Typically class 1 receptors, such as the classical steroid receptors (e.g., estrogen, androgen, glucocorticoid receptors), are ligand activated, while so-called class 2 receptors (e.g., PPARs, vitamin D receptor, thyroid receptor) function as transcriptional activators in the presence of ligand, but as repressors in the absence of ligand (Figure 1). Various post-translational modifications including phosphorylation, acetylation, ubiquitylation, and sumoylation can also modulate their activities (Anbalagan et al., 2012). Some NRs constitutively bind ligand (e.g., HNF4α binds fatty acids), and are instead gated by associated proteins or modifications (Gonzalez, 2008). Other NRs have no identified ligands, and are therefore termed orphan receptors.
Humans harbor 48 NRs including the classical steroid receptors, thyroid receptors, vitamin D receptor, and retinoic acid receptors, SF1, GCNF, ROR, COUP, which primarily govern development, immunity, and reproduction. Major regulators of lipid, glucose, and xenobiotic metabolism include PPARs, LXR, LRH, HNF4, FXR, PXR, and CAR (McKenna and O'Malley, 2010a, McKenna and O'Malley, 2010b). C. elegans boasts a remarkable 284 receptors: 269 of them represent a vast expansion and diversification of the HNF4 family (Robinson-Rechavi et al., 2005). The remaining 15 are more evolutionarily conserved, and include homologs of VITD/LXR/FXR (daf-12, nhr-8, nhr-48), SF1 (nhr-25), ROR (nhr-23), COUP (unc-55), HNF4 (e.g., nhr-64, nhr-69), Rev-Erb (nhr-85, sex-1), TLX (nhr-67), PNR (fax-1), NGF-1 (nhr-6), GCNF (nhr-91), and TR2/4 (nhr-41) (Table 1, Section 6). Notably missing from C. elegans are clear structural orthologs of the classical steroid and ecdysteroid receptors, the PPARs, thyroid receptors, as well as retinoic acid and heterodimeric receptor RXR. Nevertheless evidence suggests that analogous physiologic functions have arisen through convergent evolution (Table 2, Section 6). The expanded NR superfamily is not unique to C. elegans, but also characteristic of closely related nematodes such as C. rameni and C. briggsae (Haerty et al., 2008), as well as species more evolutionarily diverged such as Pristionchus pacificus (Dieterich et al., 2008), suggesting an ancestral role in nematode physiology.
Because of C. elegans’ cellular simplicity and powerful genetics, the study of NR signaling in the worm has provided unprecedented insights into events in vivo, from the subcellular to organismal level, from synaptic remodeling to longevity—vantages not always easily achieved in mammalian models. Since our 2006 chapter, Nuclear hormone receptors in C. elegans, a wealth of information on the C. elegans receptors has been uncovered. An emergent view is that these NRs play key roles in timers and oscillators, working as feedback regulated switches in circuits governing developmental timing, the molt cycle clock, dauer formation, longevity, and other aspects of life history. They also extensively interface with other signaling pathways to mediate fate choice and organogenesis. Finally they serve as key homeostatic regulators or switches in nutrient sensing and metabolism. In this review, we highlight the best studied of these receptors, and attempt to place them in biological context.
All animals develop through successive life stages to reproductive maturity, and ultimately age and die, collectively comprising the life history of the species. Coordinate progression through the life stages requires a precise integration of extrinsic environmental cues together with intrinsic metabolic, cellular, and physiologic processes. As ligand and nutrient responsive transcription factors, NRs play a particularly important role in orchestrating animal life history, by integrating environmental and physiologic information, coordinating metabolic and cellular events throughout the body, and triggering the succession of life stages.
C. elegans life history, like many species depends very much on environment. In favorable environmental conditions, C. elegans develops from embryo through four larval stages L1-L4 separated by molts to reproductive maturity, produces large broods of 300 animals, and then lives another 3 weeks (Byerly et al., 1976; Klass, 1977). In unfavorable conditions, such as food deprivation, animals arrest at several diapause states including the L1 diapause, the L3 dauer diapause, and an adult reproductive diapause, which are stress resistant and long lived (Cassada and Russell, 1975; Johnson et al., 1984; Angelo and Van Gilst, 2009). Upon return to ample nutrients and favorable conditions, worms will resume growth and reproduction. Several conserved NRs, including DAF-12, NHR-8, UNC-55, NHR-25, and NHR-23, function as key regulators of C. elegans life history. These NRs govern diapause stages and developmental timing circuits, catalyze transitions through life stage programs, and drive the molt cycle timer. They also often influence reproduction, metabolism, and longevity. Below we highlight their roles in these circuits.
DAF-12 is the most intensively studied NR in C. elegans and has served as an important paradigm for metazoan NR signal transduction in vivo. DAF-12 is most homologous to vertebrate farnesoid-X (FXR), liver-X, and vitamin-D receptors (Antebi et al., 2000), which regulate metabolism, development, and homeostasis in a wide variety of contexts (Table 1). Vertebrate homologs are gated by cognate bile acids (Makishima et al., 1999; Parks et al., 1999), oxysterols (Janowski et al., 1996), and vitamin-D (McDonnell et al., 1987), respectively, but also share the ability to be regulated by bile acid-like steroids, suggesting an ancestral role of such molecules (Song and Liao, 2000; Jurutka et al., 2005; Zhi et al., 2012). DAF-12 is also regulated by bile acid-like steroids, in this case called the dafachronic acids (DA) as well as cholestenoic acid, which activate transcription with high affinity (Held et al., 2006; Motola et al., 2006), and to date is the sole C. elegans receptor whose ligand has been unequivocally determined. Found in the nucleus of all somatic cells, DAF-12 regulates a wide swath of C. elegans biology, including the dauer diapause, developmental timing, metabolism, and longevity, coupling environmental and physiologic information to reproductive development, detailed below (Antebi et al., 2000).
Under food scarcity, thermal stress and overcrowding, C. elegans will arrest and enter the dauer diapause, an alternate third larval stage specialized for survival and dispersal (Cassada and Russell, 1975; Fielenbach and Antebi, 2008; see also the WormBook chapter Dauer). Dauer larvae are extremely stress resistant, sexually immature, and long lived. Yet when returned to ample food, will mature to reproductive adults, revealing incredible plasticity in regulation of reproduction and longevity. Genetic screens for mutants that affect dauer formation identified dauer-formation constitutive (Daf-c) and dauer-formation defective loci (Daf-d) that either always, or never, enter the dauer stage (Riddle et al., 1981). Genetic epistasis experiments place daf-12 at the end of the dauer pathways (Riddle et al., 1981; Vowels and Thomas, 1992; Thomas et al., 1993; Gottlieb and Ruvkun, 1994). Various daf-12 mutants show opposite phenotypes: null mutants are Daf-d and somewhat short lived, whereas ligand-insensitive LBD mutants are Daf-c and modestly long lived, indicating that DAF-12 is instructive in the dauer decision (Riddle et al., 1981; Antebi et al., 1998; Gerisch et al., 2001; Fisher and Lithgow, 2006).
The molecular and cellular architecture of dauer formation reveals an intricate coupling of environmental information to a conserved endocrine network. Environmental inputs include ascarosides as signals of population density, unidentified food cues, and temperature (Golden and Riddle, 1984a; Golden and Riddle, 1984b; Butcher et al., 2007), which are perceived and integrated by ciliated sensory neurons (Perkins et al., 1986; Bargmann and Horvitz, 1991) and transduced through cGMP signaling (Vowels and Thomas, 1992; Birnby et al., 2000). Sensory perception regulates production and neurosecretion of TGF-β (Ren et al., 1996), and insulin-like-peptides (Li et al., 2003), which work through their respective signal transduction pathways to control steroidal hormone signaling (Gerisch and Antebi, 2004; Mak and Ruvkun, 2004). This core endocrine network is modulated by a myriad of signaling inputs including serotonin (Sze et al., 2000), acetylcholine (Lee et al., 2014), neuropeptide-Y-like (Cohen et al., 2009), ALK (Reiner et al., 2008), TOR (Jia et al., 2004), AMPK (Apfeld et al., 2004), Wnt (Essers et al., 2005; Goh et al., 2012), Notch (Ouellet et al., 2008), ER stress signaling (Kulalert and Kim, 2013), and others. Despite the complexity of inputs, the essential core signaling reduces to a binary decision: that in favorable circumstances, TGF-β and insulin/IGF signaling (IIS) are upregulated and stimulate production of the DAs in steroidogenic cells (Fielenbach and Antebi, 2008). Liganded DAF-12 then prevents dauer formation, and promotes reproductive development in tissues throughout the body (Figure 2). Conversely, under adverse circumstances, TGF-β and IIS are downregulated, resulting in suppression of DA production. Unliganded DAF-12 forms a repression complex with the DIN-1/SHARP co-repressor, thereby specifying the long-lived dauer diapause (Ludewig et al., 2004; Fielenbach and Antebi, 2008). Thus, DAF-12 functions as a hormone-regulated switch governing reproduction or survival.
Apparently the dauer endocrine network has a conserved physiologic function in regulating reproduction and longevity across taxa. Both IIS and TGF-β pathways govern ecdysteroidogenesis in insect metamorphosis, and steroidogenesis in mammalian puberty (Tennessen and Thummel, 2011) (Figure 3). TGF-β, IIS, and steroidal signaling and other dauer signaling pathways have been implicated in regulation of C. elegans adult longevity (Kenyon et al., 1993; Hsin and Kenyon, 1999; Gerisch et al., 2001; Jia et al., 2002; Shaw et al., 2007). IIS regulates longevity in flies, mice and humans (Clancy et al., 2001; Tatar et al., 2001; Holzenberger et al., 2003; Willcox et al., 2008), while various steroids have been implicated to affect life span in flies and perhaps mammals (Keisala et al., 2009; Kenyon, 2010; Mooijaart et al., 2007; Simon et al., 2003). It will be important to explore whether other pathways that govern reproduction and survival also impact mammalian longevity.
Until recently relatively little was known about steroidogenic pathways in worms. Mutants were originally identified as having a similar spectrum of phenotypes as daf-12 ligand-insensitive LBD mutants, including Daf-c and retarded gonadal migration phenotypes. Initial genetic and biochemical experiments suggested that the DAs were synthesized from cholesterol (which must be obtained through the diet) through two proposed biosynthetic branches to produce active DAF-12 ligands, called Δ7-DA and Δ4-DA (Figure 4) (Motola et al., 2006; Rottiers et al., 2006). Since then, these models have undergone revision as new activities have been revealed.
The Δ7 branch is best understood (Figure 4). The first step entails the conversion of cholesterol to 7-dehydrocholesterol by the DAF-36/Rieske oxygenase (Rottiers et al., 2006; Wollam et al., 2011; Yoshiyama-Yanagawa et al., 2011). A similar Δ7 desaturation is catalyzed by the orthologous neverland/Rieske oxygenase in the first step of insect ecdysteroid biosynthesis (Yoshiyama et al., 2006; Yoshiyama-Yanagawa et al., 2011). During mammalian bile acid production, CYP7A1/cytochrome P450 hydroxylates the cholesterol backbone at the 7-position in the first step (Russell, 2003), revealing an analogous chemistry. In C. elegans, an unknown Δ5 reductase thereafter leads to the production of lathosterol, which is oxidized to the 3-keto steroid, lathosterone, by the short chain dehydrogenase DHS-16, analogous to mammalian HSD3B7 (Wollam et al., 2012). The last step in the Δ7 pathway involves sequential oxidation of the cholesterol side chain to the carboxylic acid moiety by DAF-9/cytochrome P450 (Motola et al., 2006), bearing similar chemistry to that carried out by CYP27A1 in mammalian bile acid synthetic pathways (Russell, 2003). Indeed, a compound called dafadine, which phenocopies daf-9 loss of function when fed to worms, biochemically inhibits both DAF-9 and CYP27A1 activity (Luciani et al., 2011). These studies reveal remarkable convergence in the biochemical activities of bile acid synthetic pathways from worm to human.
Less is known about nematode Δ4 production. Although proposed as an endogenous ligand derived from 4-cholestene-3-one, spectroscopic analysis failed to detect Δ4-DA in vivo, perhaps because of vanishingly small amounts or instability (Mahanti et al., 2014). The 3-hydroxysteroid dehydrogenase, hsd-1, had been proposed to work in the Δ4 branch (Patel et al., 2008), but available evidence argues against this hypothesis, and suggests instead that hsd-1 may produce alternative DA-like ligands or function as a cholesterol Δ7 desaturase specifically within the XXX neuroendocrine cells (Wollam et al., 2012; Mahanti et al., 2014). Accordingly, hsd-1 mutants exhibit phenotypes and genetic interactions distinct from other hormone biosynthetic genes with respect to dauer formation, gonadal migration, and longevity (Patel et al., 2008; Dumas et al., 2010). More recently, several new DA-related compounds have been discovered through 2D-NMR and MS analysis, including an abundant and potent Δ1,7-DA, as well as a less prevalent and less potent 3-alpha hydroxy DA (Figure 4) (Mahanti et al., 2014). In the future it will be important to determine where and how these different DAs are made, and whether the different DAs have distinct functions and transcriptomes.
Interestingly, DA production is distributed in various tissues throughout the organism. The DAF-36/Rieske oxygenase is expressed primarily within the intestine (Rottiers et al., 2006), DHS-16/3-hydroxysteroid dehydrogenase is expressed in the pharynx, head neurons, and hypodermis (Wollam et al., 2012), HSD-1 in the XXX neuroendocrine cells (Patel et al., 2008), and DAF-9/CYP27A1 in XXX neuroendocrine cells, hypodermis, and spermatheca (Gerisch et al., 2001; Jia et al., 2002). Distributed synthesis implies that there must be mechanisms involved to transport precursors from one tissue to the other. Why hormone biosynthesis is organized in this non-autonomous fashion is unknown, but speculatively it may generate tissue-specific ligands, or help coordinate the dauer decision across the organism.
Indeed, the choice between dauer and reproductive development converts noisy and variable environmental information into an all-or-none binary organismal decision. Although how this is achieved is not completely understood, it seems to rely largely on tight regulation of DA production, which involves both feed forward and feedback mechanisms that ensure this decision is robust (Gerisch and Antebi, 2004; Mak and Ruvkun, 2004; Schaedel et al., 2012). daf-9/CYP27A1 regulation is critical in this regard where its function in the neuroendocrine XXX cells and within the hypodermal syncitium apparently serves as a relay to coordinate the organismal response (Gerisch et al., 2001; Jia et al., 2002). It is hypothesized that the dauer pathways comprise a three state regulatory system to mediate this choice (Figure 5) (Gerisch and Antebi, 2004; Schaedel et al., 2012). Under favorable conditions, insulin and TGF-β signaling converge upon hormone production within the XXX neuroendocrine cell, which results in a DA signal that can be amplified in the hypodermis through upregulation of daf-9, thus locking in reproductive commitments. Moderate thermal stress, pheromone level, or modest downregulation of TGF-β, IIS, or steroidal signaling, also visibly upregulate hypodermal daf-9, presumably increasing DA to sustain reproductive development. Factors required for this upregulation include daf-12, as well as ttx-1, tax-2, pkc-1, and hsf-1 genes functioning in thermotaxis neurons, revealing a complex regulatory network linked to temperature cues (Lee and Kenyon, 2009; Monje et al., 2011; Barna et al., 2012). In unfavorable dauer inducing conditions, DA production falls below a threshold, hypodermal amplification is suppressed, and animals undergo dauer development. Shift experiments performed with DA (which promotes non-dauer development) and ascarosides (which oppositely promote dauer development) suggest that the dauer decision transpires within a critical time window of 12-18 hours post-hatch within the L1/early L2 stage and that sustained and elevated DA exposure is critical for rapid and complete reproductive development (Golden and Riddle, 1984a; Schaedel et al., 2012). Surprisingly, ascaroside treatment raised the threshold required for DA to implement reproductive program, implying a role of pheromone signaling pathways proximal or downstream of steroidal signaling. Critical tests of these models await accurate measurement of DA levels in various genotypes and tissues.
An important aspect of larval development is the correct specification of temporal fates. As C. elegans develops through the four larval stages, tissues undergo stage-specific patterns of cell division, migration, fusion, and morphogenesis. Epidermal stem cells called seams undergo asymmetric divisions at each stage, with stage-specific variations in division (Sulston and Horvitz, 1977). At the larval-to-adult transition they cease dividing, fuse, and synthesize adult alae. Vulval cells undergo stereotyped induction and division in the L3 stage, and morphogenesis during the L4 stage. The gonad undergoes cell division, outgrowth, and differentiation from L2-L4 to form two U-shaped arms harboring the maturing germ cells, spermatheca, and uterus (Kimble and Hirsh, 1979). Intestinal nuclei undergo endoreplication at each stage, with a stage-specific nuclear division in L2 (Hedgecock and White, 1985). Motorneurons also undergo changes in synaptic connectivity (Zhou and Walthall, 1998).
The so-called heterochronic genes control developmental timing, and work as temporal selectors that specify these stage appropriate patterns. Their mutation results in precocious or retarded development within specific tissues (Ambros and Horvitz, 1984). Most of the identified heterochronic regulators are evolutionarily conserved, including the first discovered microRNAs lin-4 and let-7 (Lee et al., 1993; Wightman et al., 1993; Reinhart et al., 2000) and components that regulate their maturation such as LIN-28 (Moss et al., 1997; Viswanathan et al., 2008). Remarkably the human LIN28 has been linked to the timing of puberty (Ong et al., 2009). Several NRs also play various roles in the heterochronic circuit, including DAF-12/FXR, UNC-55/COUP, and NHR-25/SF1 (Antebi et al., 1998; Hada et al., 2010; Thompson-Peer et al., 2012).
DAF-12 functions as a switch within the heterochronic circuits, promoting L2/L3 transitions. Mutants manifest retarded phenotypes in which they repeat L2 programs inappropriately at the L3 stage in seam, gonad, and intestine (Antebi et al., 1998). Such phenotypes were originally observed with high penetrance in specific LBD truncation mutants, and at low penetrance in null mutants (Antebi et al., 2000). The LBD truncation mutants, though recessive in nature, are thought to interfere with redundant or overlapping functions. Indeed, double mutant combination with other heterochronic loci affecting the L2/L3 transition strongly enhance the phenotypes of daf-12 null mutants, revealing multiple layers of regulation within the pathway (Abbott et al., 2005; Ding et al., 2005; Fielenbach et al., 2007; Hochbaum et al., 2011). Genetic epistasis experiments place DAF-12 downstream of the novel nuclear factor, LIN-14, but upstream or in parallel to LIN-28 (Antebi et al., 1998).
An important clue to DAF-12's function within the heterochronic circuits came with the observation that a triple knockout of the let-7-related microRNAs, mir-48, mir-84, and mir-241 (collectively known as let-7s), results in phenotypes similar to daf-12 mutation, that is, a reiteration of L2 seam cell division programs at the L3 stage (Abbott et al., 2005). Moreover, daf-12 mutation reduced expression levels of the let-7 family microRNAs as measured by Northern blots or qPCR, revealing that it works upstream (Esquela-Kerscher et al., 2005; Bethke et al., 2009; Hammell et al., 2009). This led to the hypothesis that DAF-12 directly transcriptionally regulates these microRNAs. Indeed, DAF-12 together with the DAs, potently transactivates the promoters of mir-84 and mir-241 in mammalian cell culture, and expression of these two microRNAs shows daf-12 and DA dependence in specific tissues in the worm (Bethke et al., 2009). Interestingly, ecdysone and estrogen receptors also regulate let-7 microRNAs in flies and mammals, respectively, revealing a possible ancestral role for steroidal control of microRNA expression by nuclear receptors (Figure 3). For example 20-hydroxy ecdysone stimulates expression of the Drosophila let-7C complex, which regulates genes required for remodeling of neuromuscular architecture during the larval-to-adult transition (Bhat-Nakshatri et al., 2009; Chawla and Sokol, 2012) and promotes temporal transitions in mushroom body neuroblast progenitors (Kucherenko et al., 2012). In mammalian MCF7 cells, estradiol treatment upregulated 8 members of the let-7 family, and the related mir-98 (Bhat-Nakshatri et al., 2009; Chawla and Sokol, 2012).
In C. elegans, the data suggest a model in which liganded DAF-12 upregulates let-7s, which in turn downregulate their target, the L2 regulatory factor HBL-1/hunchback Zn finger protein, allowing L3 reproductive programs to be expressed (Figure 6) (Bethke et al., 2009; Hammell et al., 2009). Conversely in the absence of hormone, the unliganded receptor potently represses microRNA expression during dauer formation. Thus DAF-12 works as a steroid-gated microRNA switch that functions to turn off earlier developmental programs to allow for later ones during reproductive growth, or to shut down the heterochronic timer altogether in the dauer stage. Interestingly, these same let-7-related microRNAs also feedback inhibit daf-12 through its 3’UTR, and curtail its activity for dauer formation and gonadal outgrowth (Bethke et al., 2009; Hammell et al., 2009). Similarly let-7 itself feedback inhibits DAF-12 late in larval development to facilitate the larval-to-adult transition (Figure 6) (Grosshans et al., 2005). Such feedback control may serve to temporally dampen DAF-12 activity, or help buffer developmental decisions in response to variable environmental and physiologic inputs.
In fact, mir-84 and mir-241 represent only a small fraction of DAF-12 target genes relevant to developmental progression. An analysis of DAF-12 binding sites and transcriptional activity reveals that it may regulate other heterochronic genes (lin-41, lin-42, lin-28, lit-1), other microRNAs, and genes involved in microRNA activity (ain-1, nhl-2), putative coregulators (din-1, cbp-1), as well as dauer transcription factors (daf-3, daf-16) (Shostak et al., 2004; Fisher and Lithgow, 2006; Hammell et al., 2009; Hochbaum et al., 2011), demonstrating that DAF-12 regulates key components in these circuits.
By lying at the confluence of dauer and heterochronic pathways, DAF-12 coordinates L2/L3 transitions, including the L3 dauer diapause, in response to environmental and physiologic inputs. In this capacity, it works intimately with LIN-42/Period, another heterochronic regulator that also affects dauer formation, developmental timing in epidermis, gonad, and other tissues, as well as molting (Jeon et al., 1999; Tennessen et al., 2006; Tennessen et al., 2010; Monsalve et al., 2011). LIN-42 is a homolog of Period, the circadian regulator, and is expressed in an oscillating pattern in concert with the molt cycle (Jeon et al., 1999; Monsalve et al., 2011), suggesting a clocklike function similar to its mammalian counterpart (Figure 6 and Figure 7). Notably, LIN-42 and DAF-12 appear to mutually antagonize one another's activity. For example, daf-12 retarded heterochronic phenotypes are suppressed by lin-42 mutation, and lin-42’s precocious phenotypes are often suppressed by daf-12 mutation (Tennessen et al., 2006). lin-42(+) opposes dauer formation under mild stress and works at the same step as DAF-12 during the dauer decision. lin-42 mutants are Daf-c at elevated temperatures, and lin-42(+) overexpression prevents dauer formation even in daf-12 LBD Daf-c mutants, suggesting a close association with DAF-12 (Tennessen et al., 2010). These interactions could be direct, as the two proteins physically interact in yeast two-hybrid experiments, or indirect, reflect opposing regulatory activities on target processes. It is noteworthy that several mammalian NRs bind to and are inhibited by PER2, suggesting a conserved interaction between NRs and components of the circadian clock (Grimaldi et al., 2010; Schmutz et al., 2010).
Many of the observed daf-12 heterochronic phenotypes are modulated by environmental conditions or dauer signaling (Bethke et al., 2009; Hochbaum et al., 2011; Huang and Zhang, 2011), revealing plastic and modulatory inputs into developmental timing pathways. Other processes influenced by daf-12 include the specification of chemoreceptors in sensory neurons (Nolan et al., 2002), male mate search behavior (Kleemann et al., 2008), foregut remodeling (Ao et al., 2004), and muscle arm extension (Dixon et al., 2008). These functions may be related to its roles in dauer or developmental timing. In particular extra muscle arms are induced by downregulation of DA, IIS, and TGF-β signaling (Dixon et al., 2008). Interestingly this phenotype depends specifically on a DAF-12 isoform consisting of LBD only, revealing a novel previously unknown role for this protein. Altogether these processes imply substantial plasticity in remodeling of neural and neuromuscular architecture in response to environmental and hormonal cues.
C. elegans has served as a premier model for the study of aging, and at least four major pathways have been shown to extend life span across taxa including reduced insulin/IGF, mitochondrial function, dietary restriction, and signaling from the reproductive system (Kenyon, 2010). Notably, removal of germline stem cells by laser microsurgery or genetic ablation results in an extension of C. elegans adult life span by 60% (Hsin and Kenyon, 1999; Arantes-Oliveira et al., 2002). Further removal of the somatic gonad abolishes this life span extension, suggesting that the germline makes life-shortening signals that antagonize life-lengthening signals from the somatic gonad. Regulation of life span by the reproductive system is also seen in fruit flies (Flatt et al., 2008) and perhaps even in humans (Min et al., 2012).
Regulation of longevity by the reproductive system requires the activity of at least five transcription factors, including DAF-12/FXR, DAF-16/FOXO, PHA-4/FOXA, NHR-80, and HLH-30/TFEB, which regulate lipid metabolism, fatty acid desaturation, lipolysis, autophagy, and proteasome activity to influence longevity (Figure 8) (Hsin and Kenyon, 1999; Wang et al., 2008; Goudeau et al., 2011; Lapierre et al., 2011; Vilchez et al., 2012; Lapierre et al., 2013; O'Rourke and Ruvkun, 2013). Communication between tissues suggests a hormonal mechanism is at work, and indeed, long life requires the activity of DA and DAF-12 signaling (Hsin and Kenyon, 1999; Gerisch et al., 2001; Rottiers et al., 2006; Gerisch et al., 2007; Wollam et al., 2012). In fact, life extending signals from the somatic gonad may be the DAs themselves since gonadless animals, which have normal life span, exhibit robust life span extension upon DAF-9 overexpression or DA supplementation (Yamawaki et al., 2010). Moreover, this extension is daf-12 dependent. Another key regulator in this pathway is the DAF-16/FOXO transcription factor, which mediates IIS (Hsin and Kenyon, 1999). Evidence indicates that DA/DAF-12 facilitates DAF-16/FOXO nuclear localization and activity (Berman and Kenyon, 2006 ; Gerisch et al., 2007), and that the two transcription factors cooperate to regulate longevity assurance genes in germlineless animals. DAF-12 may contribute to longevity by regulating expression of a fatty acid reductase, fard-1, as well as the lipase lips-17, which are required for longevity (McCormick et al., 2012). Surprisingly genetic manipulation of these molecules as well as the lipase lipl-4 (which is transactivated by DAF-16/FOXO) have little effect on bulk fat levels, and therefore may instead produce fatty acid signaling molecules that could work through additional nuclear receptors such as NHR-80 (see Section 4.8).
How might DAF-12 and DAF-16 cooperate to regulate longevity? Recent evidence suggests that DAF-12 regulation of let-7-related microRNAs mediates crosstalk between the two transcription factors (Shen et al., 2012). Studies by Shen and colleagues showed that germline removal results in upregulation of DA production, triggering DAF-12 dependent activation of mir-84 and mir-241, previously implicated in developmental timing circuits (Section 2.6) (Bethke et al., 2009). Importantly, these microRNAs, too, are required for the longevity of germlineless animals, as their deletion abolishes life span extension. Further molecular analysis reveals that mir-84 and mir-241 downregulate two inhibitors of DAF-16/FOXO, namely the AKT-1 kinase (Paradis and Ruvkun, 1998) and the LIN-14 heterochronic nuclear factor (Boehm and Slack, 2005), and thereby promote DAF-16/FOXO nuclear localization and activity (Figure 8) (Shen et al., 2012). Remarkably then, components of the developmental timing switch used during L2/L3 temporal transitions are co-opted to trigger a long lived adult mode. This discovery and others support the idea that components of developmental clocks are used to regulate animal life span (Gerisch et al., 2001; Boehm and Slack, 2005; Shen et al., 2012). Conceivably developmental timing components synchronize signaling between gonad and soma to ensure coordinate maturation under environmental or physiologic stress.
In ectotherms, longevity varies inversely with temperature, with animals living shorter at higher temperatures. Thermal effects on life span and other processes are ascribed to passive changes in metabolic rate, but recent evidence also suggests a regulated process that links thermotaxis and steroidal signaling (Lee and Kenyon, 2009). Thermotaxis genes ttx-1 and ttx-3 encode transcription factors that specify the fates of the temperature sensing neurons (Hobert et al., 1997; Lanjuin et al., 2003). Unexpectedly, loss of AFD thermosensory neurons through mutation or laser ablation, results in short life span at elevated temperatures (25° C), which is restored by daf-12 loss of function (Lee and Kenyon, 2009). Similar observations are seen with daf-9 hypomorphs that reduce but do not abolish DA production; such mutants are short lived at 25 °C, and daf-12 mutation suppresses this phenotype, suggesting that the unliganded receptor is life shortening in this context. Overexpression of daf-9 also restores normal life span to ttx-1 mutant animals. Altogether these observations suggest that a boost of DA production and DAF-12 activity at elevated temperatures is essential to maintain normal life. Conceivably this physiologic function is related to the activity of heat shock factor, hsf-1, which, similar to ttx-1 (Lee and Kenyon, 2009), regulates daf-9 levels in response to temperature in feedback circuits described above (Barna et al., 2012), suggesting that thermal responses are coordinated hormonally.
daf-12 mutants also show complex interactions with IIS for life span regulation. So-called daf-2 class 1 mutants robustly extend life span in a manner that is partially abrogated upon daf-12 loss, while daf-2 class 2 mutants, which are more pleiotropic, show synergistic life span extension upon daf-12 depletion (Gems et al., 1998), an interaction that is hermaphrodite specific (McCulloch and Gems, 2007). Several other loci, such as let-60/Ras and hormone biosynthetic genes evoke a similar complex interaction, but the underlying molecular mechanism remains obscure (Nanji et al., 2005; Dumas et al., 2013). daf-12 also functions in hormesis-induced longevity. When animals are subjected to moderate brief pulses of heat stress, they acquire subsequent thermotolerance and longevity, which depends on the activity of daf-12, daf-16, daf-18, and other factors (Cypser et al., 2006). Lastly, DAF-12 has been shown to contribute to precocious aging and somatic collapse of hermaphrodites that have been mated by males (Shi and Murphy, 2013). Mated hermaphrodites shrink and die early due to signals from sperm and seminal fluid: the shrinkage phenotype is mediated by DA/DAF-12 pathways, while the short-lived phenotype largely arises from induction of INS-7 and inhibition of DAF-16/FOXO.
Remarkably, DA/DAF-12 signaling has been conserved in nematodes that are diverged from C. elegans by over 150 million years, and functions similarly as an important regulator of life history traits and phenotypic plasticity. The free-living nematode Pristionchus pacificus has co-opted the DA/DAF-12 module, not only to regulate dauer formation, but also to select alternative mouth dimorphisms involved in predatory feeding (Ogawa et al., 2009; Bento et al., 2010). As with C. elegans, germline elimination extends P. pacificus life span, and in addition enhances innate immunity in a manner dependent upon daf-12 and daf-16 (Rae et al., 2012). The DA/DAF-12 module has also been deployed to regulate the infective stage of parasitic nematodes, which is comparable to dauer. Treatment of the parasites Strongyloides stercoralis, Acylostoma spp, and Strongyloides papillosus with Δ7-DA variously prevents formation of the L3 infective stage or promotes exit from the infective stage, much like DA prevents dauer formation or promotes dauer exit (Ogawa et al., 2009; Wang et al., 2009). Several nematode DAF-12s are transactivated by DA and cholestenoic acid in cell culture. Such activity is confirmed in the crystal structure of Δ7-DA bound to the LBD of DAF-12 from S. stercoralis, which reveals that the receptor forms a canonical three layered sandwich comprised of 13 alpha helical regions and three beta strands typical of NRs (Wang et al., 2009). The orientation of ligand and contact residues within the LBD pocket most resembles the way in which bile acids bind to FXR, implying that DAF-12 and FXR are biochemical orthologs (Zhi et al., 2012). Significantly, many of the parasitic nematodes lack endogenous DAs as well as a daf-9/CYP27A1, suggesting they rely on host-produced bile acid-like steroids to stimulate exit from the infective stage.
Altogether these studies indicate the vast potential for exploiting the DA/DAF-12 axis to combat parasitic disease. For example, DA-like molecules could be used to trigger precocious exit from the infective stage in the wrong environment, or DA-inhibitors could be used to prevent exit in compatible hosts.
NHR-8 is the closest relative to DAF-12 in C. elegans, and homologous to vertebrate LXR, FXR, PXR, and VITD receptors. Expressed primarily in the gut, NHR-8 controls production of the bile-acid like DAs and thereby DAF-12 activity through regulation of DAF-36/Rieske oxygenase and cholesterol disposition (Magner et al., 2013). Mutants display lower levels of daf-36 mRNA and protein, and make less of the DAF-36 metabolic product, 7-dehydrocholesterol, as well as DA itself. Consistent with a role in DA metabolism, mutants enter the dauer diapause constitutively on low cholesterol or at elevated temperature (27 °C), and have reduced expression of the DAF-12 target gene mir-241. By controlling the first step in DA production, NHR-8 may thereby regulate cholesterol, bile acid flux, and DAF-12 activity. These functions are analogous to its vertebrate relative, LXR, which regulates the first step of bile acid synthesis and affects activity of FXR (Calkin and Tontonoz, 2012), the homolog of DAF-12 (Figure 4). Steady state levels of cholesterol are lower in nhr-8 mutant embryos as measured by uptake of NBD-cholesterol, suggesting a deficiency in transport or metabolism in the germline. Indeed, several phenotypes manifest under low cholesterol (e.g., shortened life span, FA desaturation defects) or no cholesterol conditions (larval arrest). Transcriptome analysis of nhr-8 mutants under low cholesterol conditions reveals altered expression of genes involved in fatty acid desaturation, lipolysis, transport and vitellogenesis, as well as those functioning in host defense and life span regulation (Magner et al., 2013). nhr-8 mutants have reduced mRNA expression of the fatty acid desaturases, fat-5 and fat-7, and correspondingly higher levels of saturated and lower levels of mono- and polyunsaturated FA. Regulation of FA desaturation and apolipoprotein production are also features of the vertebrate LXR (Calkin and Tontonoz, 2012). Interestingly, the Drosophila DHR96, a close relative of NHR-8 and DAF-12, also regulates fatty acid and cholesterol metabolism (Horner et al., 2009; Sieber and Thummel, 2012). Mutants are sensitive to cholesterol deprivation, and have dysregulated cholesterol balance. DHR96 has been shown to bind to cholesterol, although it is not known if this serves as an activating ligand, inverse agonist, or competency factor (Horner et al., 2009). Although the NHR-8 ligand is unknown, it is surmised to be a sterol derivative based on its sterol dependent phenotypes.
The NR COUP is highly conserved in evolution and functions in neural and cardiovascular development, pituitary and reproductive function, and metabolism (Lin et al., 2011). The C. elegans homolog unc-55 regulates synaptic remodeling of GABAergic motoneurons during the L1 stage, through conserved developmental timing pathways (Zhou and Walthall, 1998; Thompson-Peer et al., 2012). Specifically, embryonically born DD motoneurons switch their synaptic outputs from ventral onto dorsal muscles during the L1 stage, while larval-born VD neurons do not. Normally, unc-55(+) prevents VD neurons from undergoing synaptic remodeling; in null mutants larval VD neurons remodel inappropriately, similar to the embryonic DDs. Consistent with a cell autonomous role, unc-55 is expressed in the VDs during larval development. Additionally, it resides in AS and other motor neurons (Zhou and Walthall, 1998), suggesting that other roles remain to be discovered.
How might unc-55 regulate synaptic switching? Apparently DD switching is driven by the heterochronic regulator hbl-1/hunchback, which is repressed by unc-55 in the VDs (Thompson-Peer et al., 2012). In C. elegans hbl-1 loss-of-function mutants, DD remodeling is delayed, whereas in microRNA mir-84 mutants, which conversely cause hbl-1 overexpression, DD remodeling is precocious. Given the regulatory relationships of daf-12, mir-84, and hbl-1 in seam cells described above, it seems plausible that steroid signaling too might function in synaptic remodeling. Transcriptional profiling of unc-55 reveals differential expression in a large number of genes compared to wild type, including the Iroquois homeodomain protein IRX-1, which also regulates DD remodeling (Petersen et al., 2011). The UNC-55/HBL-1 regulatory interaction strikingly resembles those seen in Drosophila where seven-up/COUP temporally regulates hb/hunchback during neurogenesis (Kanai et al., 2005; Benito-Sipos et al., 2011).
We now turn our attention to NHR-25, NHR-23, and other receptors that function in another type of timing device, the molting clock. NHR-25 is a highly conserved NR that plays critical roles in development and metabolism. Its closest relative in Drosophila, Ftz-F1, is involved in early embryonic patterning, larval molting, and metamorphosis (Ou and King-Jones, 2013). The mammalian homolog, SF-1, is implicated in steroidogenesis, hypothalamic, adrenal and gonadal development, and sex determination (Ferraz-de-Souza et al., 2011). Another homolog, LRH, functions in cholesterol, bile acid, glucose, and fatty acid metabolism. It also modulates pluripotentency of embryonic stem cells, and has a role in inflammation and stem cell renewal in the gut (Fernandez-Marcos et al., 2011)
Like its fly counterpart, C. elegans NHR-25/SF-1 coordinates epidermal morphogenesis, differentiation, and molting. nhr-25 is expressed early in embryogenesis first within the E lineage, which gives rise to the gut, and thereafter in hypodermal cells, pharyngeal and rectal epithelial cells, somatic gonad, and germline. During embryogenesis, mutation of nhr-25 or knockdown by RNAi results in lethality at the two-fold stage at the initiation of elongation (Asahina et al., 2000; Gissendanner and Sluder, 2000). Although epidermal cells are specified and divide normally, they fail to fuse properly during epidermal morphogenesis resulting in ventral closure defects. During larval development, the epidermal seam cells fail to reestablish contacts at the lateral midline and lose their spindle shape, possibly leading to ectopic cell division in the adult and poor alae formation (Chen et al., 2004; Silhankova et al., 2005). Knockdown of nhr-25 within the seam cells is sufficient to recapitulate these defects, suggesting a cell autonomous role (Hajduskova et al., 2009; Hada et al., 2010). Mutants also have additional defects in vulva division, fusion, and morphogenesis (Chen et al., 2004). In this context, correct vulval specification depends on dampening of NHR-25 transcriptional activity by sumoylation (Ward et al., 2013).
NHR-25 functions most prominently within the C. elegans molt cycle (Figure 6 and Figure 7). Each of the four successive larval stages (L1-L4) is punctuated by molts. The molt cycle begins with synthesis of the new cuticle, an intermolt period characterized by feeding, activity, and growth, followed by a brief hiatus of sleep-like inactivity, termed lethargus, during which the new cuticle is deposited and the old one shed, and then emergence into the new intermolt. This process takes approximately 8-10 hours for each stage (Monsalve and Frand, 2012). During the larval molts, nhr-25 mutants are unable to shed the old cuticle and often bear residual cuticle stuck to mouth, vulva, and rectal areas (Asahina et al., 2000; Gissendanner and Sluder, 2000). nhr-25 mRNA and protein levels oscillate, with peak mRNA levels at the molt (Figure 7) (Gissendanner et al., 2004; M. Horn and A. Antebi, unpublished). Several key genes implicated in ecdysis and epidermal differentiation are regulated by nhr-25, including the angiotensin converting enzyme homolog acn-1, the nematode-specific gene mlt-10, the collagenase nas-37, and the amyloid precursor protein homolog apl-1 (Brooks et al., 2003; Davis et al., 2004; Frand et al., 2005; Hornsten et al., 2007; Hada et al., 2010; Meli et al., 2010; Wiese et al., 2010).
Recently, it has been suggested that nhr-25 also acts in the heterochronic circuit. nhr-25 mutants display defects reminiscent of retarded heterochrony, including shallow adult alae formation and delayed male tail retraction (Hada et al., 2010; Nelson et al., 2011). Moreover, nhr-25 depletion suppresses the precocious heterochronic phenotypes of hbl-1, lin-41, and lin-42 mutants, although its interactions with other heterochronic mutants suggest a more complex network (Hada et al., 2010). nhr-25 RNAi also suppresses the supernumerary molt phenotype of let-7 mir-84 double mutants, suggesting that let-7s downregulate nhr-25 at the L4/adult transition, leading to a cessation of molting (Hayes et al., 2006; Hada et al., 2010). In accord with this idea, the nhr-25 3’UTR contains predicted let-7 binding sites, but it remains to be seen whether regulation is direct. The observation that nhr-25 works at the convergence of the molt cycle and the developmental timer strongly suggests intimate interactions with lin-42/period, a gene that also affects both processes (see Section 2.14) (Jeon et al., 1999; Monsalve et al., 2011).
Molting is also controlled by another key nuclear hormone receptor, NHR-23, a homolog of Drosophila DHR3 and the mammalian ROR (Figure 7), which are all components of biological clocks. During the Drosophila larval molt cycle and prepupal to pupal transition, the ecdysone receptor stimulates Ftz-F1β, which in turn regulate DHR3 within ecdysone signaling cascades (Ou and King-Jones, 2013). The mammalian RORα is a central component of the circadian clock, positively regulating BMAL and the NR REV-ERB (Ranhotra, 2012). In turn, REV-ERB feedback inhibits ROR, contributing to the periodicity of circadian rhythms. ROR also functions in cerebellum development, immunity, and lipid, cholesterol, and glucose metabolism.
Similar to nhr-25, nhr-23 null mutants arrest at the comma or three-fold stage of embryogenesis (Kostrouchova et al., 1998; Kostrouchova et al., 2001). Hatched L1 larvae are dumpy, indicating morphologic or elongation defects. Also similar to nhr-25, nhr-23 RNAi knockdown in larvae results in defects in molting and alae formation (Kostrouchova et al., 2001). nhr-23 is expressed predominately in epidermal tissues, including seam cells, P-ectoblasts, and hypodermal cells. Expression of nhr-23 mRNA oscillates with the molt cycle, but with opposite phase to nhr-25, peaking during the intermolt (Figure 7) (Gissendanner et al., 2004), though whether protein expression also oscillates has yet to be determined. The reciprocal relationship between nhr-23 and nhr-25 expression suggests that they could regulate one another in a feedback loop, but this has not been directly tested. Surprisingly, the reciprocal regulatory relationship of ROR and REV-ERB seen in the mammalian circadian clock is not seen in the C. elegans counterparts, NHR-23 and NHR-85. Although nhr-85 expression varies with the molt cycle, nhr-85 depletion does not result in molting defects except at the dauer molt. nhr-85 also affects egg laying (Gissendanner et al., 2004). Another REV-ERB homolog, sex-1, specifies the hermaphrodite sexual fate. It serves as a dose dependent X chromosome signaling element that antagonizes autosomal signaling elements in the promoter of xol-1, a key sex determination gene (Carmi et al., 1998; Farboud et al., 2013).
Transcriptome profiling reveals that NHR-23 regulates expression of various genes implicated in molting including, cuticle collagens (dpy-2,-3,-5,-7,-8,-10), hedgehog-related genes (wrt-1,-2,-3, grd genes), patch-related genes (ptc-3), and molting genes (mlt-8,-9,-10,-11) (Kouns et al., 2011). Similar to nhr-25, nhr-23 also affects expression of a number of genes functionally implicated in molting, including acn-1 and mlt-10 (Frand et al., 2005; Kouns et al., 2011). Curiously, several genes involved in DA biosynthesis, including dhs-16 and daf-9, also appear to be nhr-23 regulated, perhaps relevant to a role in the dauer molt (Gissendanner et al., 2004; Kouns et al., 2011).
Other NRs may also be tied to the molt cycle. The HNF4-like family member, nhr-60, is regulated by NHR-23 within seam cells (Simeckova et al., 2007). RNAi knockdown of nhr-60 results in embryonic lethality, poor ventral closure, and seam cell defects similar to nhr-23 and nhr-25 mutants, suggesting nhr-60 could work in transcriptional cascades driving early morphogenetic events. The DHR78/TR2 homolog, nhr-41, and possibly daf-12, are expressed in a cyclical pattern along with the molt cycle, as measured by mRNA (Figure 7) (Gissendanner et al., 2004; Merris et al., 2007). However, DAF-12::GFP does not overtly oscillate at the protein level, and mutation of these receptors does not explicitly affect molting per se, though both affect the dauer molt and fat accumulation (Gerisch et al., 2001; Gissendanner et al., 2004; Arda et al., 2010).
NHR-40 is another receptor required for late embryonic morphogenesis, elongation, and proper muscle formation and motility. Accordingly, it is expressed primarily in the pharyngeal, body wall, and sex muscles, but also in a handful of neurons (Brozova et al., 2006). Proteomic analysis of nhr-40 mutants reveals changes in the level of proteins enriched in muscle function and metabolism (Pohludka et al., 2008). Given its phenotypes and expression pattern, nhr-40 will likely strongly interact with genes involved in muscle biogenesis, morphogenesis, and attachment, such as the paralyzed at two-fold (Pat) mutants (Williamsa and Waterston, 1994).
Although the molt cycle is apparently an adaptation of the Ecdysoa clade of animals, the study of its circuitry could very well shed light on mammalian circadian rhythms and sleep. As mentioned earlier, components of the molt cycle include those implicated in circadian rhythms in mammals, NHR-23/ROR and LIN-42/Period, suggesting an ancient origin for these clocks (Figure 6 and Figure 7). The short C-terminal lin-42a isoform is specifically implicated in molting. It affects the length of lethargus and the molt cycle, the execution of the molt, the expression of molting genes, and seam cell morphology, functionally resembling nhr-23/25. Accordingly, lin-42a is expressed cyclically with peaks at the molt, while the longer lin-42bc isoforms, which specifically affect developmental timing, peak during the intermolt (Jeon et al., 1999; Tennessen et al., 2006; Monsalve et al., 2011). Thus regulatory cascades used in mammalian circadian timers may have been co-opted by the molt cycle, and components identified in either pathway may inform the other. Another interesting aspect of the molt cycle that resembles sleep of mammalian circadian rhythms is lethargus, a quiescent sleep-like state, during which the animal lays down the new cuticle and sheds the old (Raizen et al., 2008). A molecular genetic dissection of genes that affect lethargus in C. elegans so far include lin-42a/period, cGMP (egl-4/protein kinase G), EGF, Notch signaling, and others (Van Buskirk and Sternberg, 2007; Raizen et al., 2008; Monsalve et al., 2011; Schwarz et al., 2011). Study of such molecules may help illuminate related conserved pathways involved in metazoan sleep.
A major unresolved question is whether the C. elegans molt cycle is hormonally regulated. The broad synchronization of events across tissues suggests so, but no hormone has yet been found. Although homologs of DA hormone biosynthetic genes have been implicated in insect ecdysteroid metabolism (Yoshiyama et al., 2006; Guittard et al., 2011), the worm genes do not obviously affect molting. Moreover, C. elegans lacks ecdysteroids and the ecdysteroid receptor. Nor have high throughput RNAi screens for molting defects identified obvious steroidogenic enzymes (Frand et al., 2005). Nevertheless, C. elegans molting is contingent upon dietary cholesterol, and several genes inferred to be involved in cholesterol transport, including the megalin homolog lrp-1, hedgehog-related and patch-related receptors, and the APP homolog apl-1, affect molting (Yochem et al., 1999; Zugasti et al., 2005; Hornsten et al., 2007; Wiese et al., 2010). Intriguingly, various 7-oxysterols reportedly bind mammalian ROR as inverse agonists (Wang et al., 2010); conceivably similar molecules could regulate NHR-23. Alternately C. elegans molting may be regulated by other lipids, since mammalian SF-1 binds phospho- and sphingolipids (Krylova et al., 2005; Urs et al., 2007; Lee et al., 2011), and C. elegans NHR-25 reportedly binds to fatty acid-phosphoinositides (Mullaney et al., 2010). These could also serve as molting hormones that link fatty acid availability to developmental advance. Clearly identifying molting hormones will remain a major important challenge for the future.
During development, multipotent cells progress through a succession of states to build cell types, organs, tissues, and integrated systems. Cells choose between alternate fates of more restricted potential in response to intrinsic and extrinsic cues. These fate choices often entail integrating inputs from growth and signal transduction pathways that impart positional and temporal information. Signaling inputs also serve to detect, quantify, and relay information about the state of the system, and often work combinatorially to specify fate choice. Ultimately, these converge on a coterie of transcription factors whose transcriptional output determines cell type. As key transcriptional regulators responding to physiologic and environmental input, NRs also function as important modulators of cell fate and organogenesis. Below, we highlight the interactions of nhr-25, nhr-67, and nhr-6 with various signaling pathways during morphogenesis.
The wingless signaling pathway (Wnt) is used at various points in development to specify asymmetric patterns of division and differentiation. In C. elegans, nhr-25 both antagonizes and enhances non-canonical Wnt signaling in the somatic gonad and epidermis, respectively (Asahina et al., 2006; Hajduskova et al., 2009). During C. elegans gonadogenesis, two primordial somatic gonadoblasts each give rise to distal and proximal blast cells that organize the bilobed arms of the gonad along the distal-proximal axis. Migratory distal tip cells coordinate outgrowth of the gonad, while proximal gonadal cells give rise to spermatheca and uterine tissues (Kimble and Hirsh, 1979). Wnt signaling components primarily help specify the gonadal distal fate. Reduction of function mutations in pop-1/TCF, the β-catenin homologs sys-1 and wrm-1, and the map kinase homolog lit-1 result in loss of the distal tip cells, resulting in a stunted gonad and sterility (Miskowski et al., 2001; Siegfried and Kimble, 2002; Siegfried et al., 2004). Conversely, nhr-25 loss results in a defect in the specification of proximal fate and inappropriate promotion of the distal fate, resulting in improperly formed spermatheca and uterine tissues, excess distal tip cells, and a tumorous germline (Asahina et al., 2006). Consistent with antagonistic behavior between nhr-25 and wnt signaling, double mutant combinations result in mutual suppression and more normal gonadal development.
This genetic interaction corresponds to physical and functional interactions of NHR-25 with the β−catenin homologs controlling transcriptional events. WRM-1 protein inhibits the transcriptional activity of NHR-25 in cell culture, while SYS-1 enhances it (Asahina et al., 2006). Moreover, NHR-25 inhibits the transcriptional activity of the POP-1/SYS-1 complex. This has led to a model in which NHR-25 in presumptive proximal cells inhibits the activity of the POP-1/SYS-1 complex to suppress distal fates, and works together with SYS-1 to promote proximal fates (Figure 9) (Asahina et al., 2006). Conversely, NHR-25 in presumptive distal cells is inhibited by WRM-1, where the POP-1/SYS-1 complex can then specify distal fates. The antagonistic interaction with Wnt signaling appears to be tissue specific, since nhr-25 works synergistically with Wnt signaling to specify asymmetric cell fates in the epidermal T cells (Hajduskova et al., 2009). This behavior fits with observations in mammals where SF-1 works in concert with Wnt signaling to regulate target gene expression (Salisbury et al., 2007).
Evidence suggests that NHR-25 also physically and functionally interacts with other transcription factors for epidermal phenotypes. These include the homeobox proteins, CEH-39, involved in regulating Pn.p fusion events, NOB-1, involved in specification of posterior regions of the animal (Chen et al., 2004), as well as the NR NHR-91/GCNF (Ward et al., 2013). These observations suggest that NHR-25 may serve as a competency factor for other transcription factors in a number of contexts.
NHR-67 is an ortholog of the highly conserved TLL/TLX tailess NR. During Drosophila embryogenesis tailless inhibits segmentation, and promotes terminal fates in the posterior of the embryo (Gui et al., 2011). Later in development it regulates neurogenesis and neural stem cell renewal. Similarly, mammalian TLX affects nervous system and visual development, embryonic and adult neural stem maintenance, and prevents glial differentiation. It is thought that TLX/TLL are orphan receptors working principally as transcriptional repressors (Gui et al., 2011). C. elegans nhr-67 is expressed in late stage embryos, in epidermal cells including hyp7 and vulval cells, the excretory cell, somatic gonadal cells including the hermaphrodite anchor cell, uterine cells, and the male linker cell, as well as the male tail, and a handful of head neurons. Null mutations result in L1 arrest and lethality along with cuticle malformations and bulges, suggesting a role in embryonic morphogenesis (Gissendanner et al., 2004; Verghese et al., 2011). Additionally, animals exhibit cystic canal defects, which may be another cause of lethality (Sarin et al., 2009). RNAi knockdown or partial loss of function in larvae results in egg laying and protruding vulva phenotypes at the gross level (Gissendanner et al., 2004; Verghese et al., 2011). Detailed examination of egg-laying (Egl) and protruding vulval (Pvul) defects reveals that they arise from altered fate specification in vulva and somatic gonad, suggesting a coordinate role in late stage aspects of reproductive maturation (Fernandes and Sternberg, 2007; Verghese et al., 2011).
Specific nhr-67 regulatory interactions and phenotypes suggest a complex late larval role in vulval morphogenesis in which the receptor regulates key growth factor signaling pathways. Although vulval cell lineages are grossly normal in nhr-67 RNAi treated animals, specific fusion events in primary derived (P6.p) vulE and vulF cells are aberrant (Fernandes and Sternberg, 2007). In vulF cells, nhr-67 positively regulates the primary fate marker lin-3/EGF and simultaneously inhibits secondary fate markers, egl-17/FGF and egl-26/LRAT. In secondary vulval cell derivatives, nhr-67 positively regulates zmp-1/zinc matrix metalloproteinase in vulA and pax-2 and egl-17/FGF in vulD cells. Thus nhr-67 can activate or repress the same gene (e.g., egl-17/FGF) depending on cell type. nhr-67 is expressed in a dynamic pattern during vulval morphogenesis and exhibits complex genetic and regulatory interactions with a number of other transcription factors in these tissues, namely lin-11/LIM, cog-1/NKX6.1/6.2, and egl-38/PAX2. In particular, nhr-67 and cog-1 appear to have mutually antagonistic inhibitory interactions in this context (Fernandes and Sternberg, 2007). Further understanding of such interrelationships may give insight into the complex regulatory networks governing organ morphogenesis.
nhr-67 also functions in uterine morphogenesis, which is closely coordinated with vulval morphogenesis. Again nhr-67 is expressed in a highly dynamic pattern within various cells of somatic gonad and uterus (Verghese et al., 2011). An important insight into nhr-67 function came from the realization that it affects several binary fate decisions governed by lin-12/Notch and its ligand lag-2/delta, including the anchor cell (AC)/ventral uterine (VU) blast cell decision, and the fates of uterine pi and uterine seam junction cell fusions (Verghese et al., 2011). Consistent with a role in regulating Notch, expression of both LIN-12/Notch and its ligand are down in various uterine cell types in nhr-67 mutants. Consequently, mutant animals have two AC cells, that is, VU cells are transformed to AC, a phenotype similar to Notch loss of function (Seydoux and Greenwald, 1989). The AC fate is nevertheless weakly determined, since several AC markers such as zmp-1 are not expressed. Because the AC further induces fates amongst vulva precursor cells, it seems likely that some of the observed vulval defects are a secondary consequence of the AC/VU decision. Interestingly, mammalian tailless regulates neural stem cell cycling, which also is influenced by Notch (Gui et al., 2011). Conceivably this regulatory circuitry is conserved throughout evolution.
A related gonadal function of nhr-67 lies in the developmental timing of male gonadal morphogenesis (Kato and Sternberg, 2009). During larval development the male somatic gonadal linker cell (LC) leads a column of proliferating germ cells through stereotyped outgrowth. Shortly after its birth, the LC migrates anteriorly during the L2 stage, dorsally by the L2 molt, posteriorly and then ventrally during the L3, and back to the tail tip during L4 (Kimble and Hirsh, 1979). These migrations are subject to both positional guidance cues as well as temporal control. Several transcription factors regulate various segments of the migratory path. In particular, nhr-67 is expressed in the LC and responsible for promoting ventral and posterior migrations during the L4 stage (Kato and Sternberg, 2009). In nhr-67 RNAi treated animals, these migrations fail, and the LC often remains dorsal. Ventral migration depends on expression of the unc-40 and repression of the unc-5 guidance receptors, which are respectively attracted to or repelled by ventral netrin cues (Hedgecock et al., 1990). nhr-67 functions to repress unc-5 expression in the LC, and activate zmp-1 at the same time. The general role of nhr-67 seems primarily to affect the temporal aspects of the LC migration program, including the timed polarization of the LC in the L4 stage. Surprisingly, the corresponding migratory cell in hermaphrodites, the distal tip cell, does not express nhr-67, nor does it show a phenotype in the nhr-67 knockdown, suggesting a male specific role. Null mutations in daf-12 also affect late stage migrations of the LC (Antebi et al., 1998). It would be of interest to understand the regulatory relationship between nhr-67 and daf-12 in this context.
Expression profiling of microdissected male LCs, comparing nhr-67 mutants to wild type, reveals that a remarkable number of genes are expressed within the migrating cell, including a multiplicity of transcription factors, cytoskeletal elements, and ion channels (Schwarz et al., 2012). Upon knockdown, several of these genes perturb migration, including hlh-8/Twist homolog, crh-2/CREB homolog, sphk-1/sphingosine kinase, the conserved transmembrane mechano- and cold sensor trpa-1, and tandem pore potassium ion channels. Future exploration of the mechanism underlying the activity of these various genes in the migration program should prove interesting.
Like its vertebrate relatives, nhr-67 has a prominent role in the specification of neuronal cell fates. The best-studied case involves the specification of the bilateral ASER/L neurons, a pair of head chemosensory neurons responsive to soluble attractants. Their fates are first specified by the zinc finger transcription factor che-1, which turns on genes specific for ASE function (Uchida et al., 2003). Thereafter ASEL and ASER become asymmetric and functionally distinct, by virtue of an elaborate bi-stable feedback loop that restricts expression of left/right markers (Johnston and Hobert, 2003; Sarin et al., 2007). Among the many players, the nkx6.1/6.2 transcription factor cog-1 specifies the ASER fate, while its downregulation by the lsy-6 microRNA species the ASEL fate.
Notably nhr-67 functions at both steps to specify ASEL/R fate. First, it regulates expression of che-1, thereby functioning in the initial specification of the ASE cell. Second, it regulates asymmetry by promoting the ASER fate and repressing the ASEL fate (Sarin et al., 2009). nhr-67 facilitates ASER by direct transcriptional activation of cog-1. Consequently, mutations in nhr-67 often result in the expression of ASEL markers in the ASER cell, and loss of ASER markers in ASER (Sarin et al., 2009). nhr-67 also regulates the fate of a number of other amphid neurons, including ASK and ASJ but its role in these cells is less well defined. Interestingly, L/R symmetry breaking events in the nervous system deploy Notch signaling at distinct points earlier in embryogenesis (Cochella and Hobert, 2012). It remains to be seen whether nhr-67 regulates Notch signaling in this context.
A close relative of NHR-67, FAX-1 is a conserved homolog of the vertebrate PNR implicated in the specification of photoreceptor rods and cones of the retina. Mutations in this receptor lead to night blindness and eye degeneration (Schorderet and Escher, 2009). In C. elegans, fax-1 is responsible for axon pathfinding and neurotransmitter expression in the AVK interneurons, and helps specify various terminal interneuron identities in conjunction with the UNC-42 homeodomain protein (Much et al., 2000; Wightman et al., 2005).
NHR-6 is a homolog of the highly conserved NOR1/Nurr77/Nurr1 subgroup of receptors. In mammals the three paralogs function in diverse processes including immediate early response, neural development, specification of midbrain dopaminergic neurons, immunity and inflammation, atherogenesis, adrenal steroidogenesis, and glucose and mitochondrial metabolism (Zhao and Bruemmer, 2010). DHR38 is the Drosophila homolog and functions in molting and metamorphosis, as well as glycogen storage and carbohydrate metabolism (Ruaud et al., 2011). These receptors are thought to work in a ligand independent fashion.
nhr-6 is expressed in a single pair of neurons in the head, possibly the ASI, as well as within dorsal somatic gonadal cells that later give rise to the hermaphrodite spermatheca (Gissendanner et al., 2004; Gissendanner et al., 2008). Consistent with a role in this organ, mutants have abnormal spermathecal morphogenesis, resulting in an Egl phenotype and ovulation defects. Spermathecae lack about half their cells, and are missing the uterine/spermathecal valve, suggesting cell lineage defects arising from various gonadoblasts (Gissendanner et al., 2008). Accordingly, various spermathecal markers are misexpressed or down, including cog-1/nkx6.1 and let-502/rho associated kinase. The neuronal function of nhr-6 remains unknown, but possible localization in ASI may suggest a role in dauer formation, sensory signal transduction, or endocrine control. Recently NHR-6 has been shown to physically and functionally interact with c-jun during spermathecal development. (Gissendanner et al., 2013). Moreover, two-hybrid data suggest physical interaction with several components of wingless signaling (Li et al., 2004). It would be of interest to investigate the in vivo relevance of such interactions in the future, as well as possible interaction with NHR-25 and NHR-67. nhr-6 also varies with the molt cycle, but the significance of this is not clear (Figure 7) (Asahina et al., 2000; Gissendanner and Sluder, 2000).
From juvenile stages to adult, animals must sense nutrient levels and modulate growth rates, maturation, and reproductive output accordingly. Nutrient levels must be closely monitored, balanced, and coordinated with biosynthetic and energy demands of the organism. NRs are crucial nutrient sensors involved in detecting key metabolites and coupling their flux to metabolism, energy homeostasis, growth, and reproduction. They play a particularly important role in lipid and carbohydrate metabolism, and function in circuits governing their synthesis, transport, storage, and breakdown. Several C. elegans receptors are implicated in coupling nutrient levels to growth, and metabolism, including nhr-91, nhr-62, nhr-23, and nhr-25. Other NRs may more specifically regulate fat and glucose metabolism, including nhr-49, nhr-80, nhr-64, nhr-69, nhr-76, and others. Finally, some NRs, such as nhr-49 and nhr-114, are also involved in maintaining metabolic balance, and detoxifying endo- and xenobiotics to preserve organismal homeostasis. Below we discuss the role of the various NRs in these circuits.
NHR-91 is a homolog of the mammalian GCNF (germ cell nuclear factor), a transcriptional repressor that restricts pluripotency gene expression during stem cell differentiation in embyrogenesis (Wang and Cooney, 2013). Although little is known about nhr-91 function in the worm, recent studies suggest a connection between nutrient sensing and stem cell quiescence and progression during the L1 diapause (Kasuga et al., 2013). When L1 larvae are starved, they arrest development and shut down blast cell division, entering a stress and starvation resistant L1 diapause (Johnson et al., 1984). A number of loci have been found that inappropriately stimulate L1 progression of blast cells (M mesoblast, P ectoblasts) under nutrient deprivation (Baugh and Sternberg, 2006; Fukuyama et al., 2006), including loss of function of microRNA mir-235 (Kasuga et al., 2013). Evidently, one of the targets of mir-235 downregulation during starvation is nhr-91, which harbors mir-235 binding sites in its 3’UTR. Consistent with a role in this pathway, nhr-91 expression is downregulated in the L1 diapause, and nhr-91 loss alleviates mir-235 mutant defects, thereby preventing P ectoblast migration, and halting the onset of markers of molting and developmental progression under starvation (Kasuga et al., 2013). These studies suggest that nhr-91 downregulation is important for blast cell quiescence under nutrient limitation, and conversely, that its upregulation is associated with stem cell progression. Knockdown of nhr-91 by RNAi also reportedly gives rise to molting and vulval defects, although it is unclear if these phenotypes are seen in the deletion mutant (Zhao et al., 2004).
Dietary restriction (DR), a modest reduction of food intake without malnutrition, leads to health benefits and extends the life span of yeast, worms, flies, rodents, and perhaps primates (Mair and Dillin, 2008; Mattison et al., 2012; Colman et al., 2014). Several paradigms of DR in the worm extend life span, including bacterial dilution (Klass, 1977) and mutation of eat-2 (Lakowski and Hekimi, 1998), which affects a nicotinic acetylcholine receptor subunit causing worms to ingest less food (McKay et al., 2004). Recently, the C. elegans HNF4α related receptor, NHR-62, has been shown to extend lifespan under DR, providing the first causal evidence of NR control of DR-induced longevity (Heestand et al., 2013). Deletion of nhr-62 largely abolished the longevity of eat-2 mutants, and dampened life extension by bacterial dilution at lower nutrient ranges. nhr-62 mutants also perturbed DR-induced changes in triglyceride accumulation, fat metabolism, and autophagy. In particular, autophagy—the turnover of cytosolic and organellar components through engulfment and lysosomal degradation—is induced under nutrient limitation and has been shown to be required for DR induced longevity (Hansen et al., 2008). NHR-62 affects autophagy, since mRNA levels of the LC3 homolog, lgg-1, as well as LGG-1::GFP puncta are down in nhr-62 mutants under DR. Transcriptome analysis reveals approximately 600 genes regulated by NHR-62 under DR, including various kinases, phosphatases, genes involved in lipid transport and metabolism, amine metabolism, and chromatin structure (Heestand et al., 2013). These findings raise the possibility that DR mediated longevity could be modulated by a NHR-62-dependent metabolite or ligand, and suggest various biological processes that could contribute to the health and longevity benefits of DR. Notably HNF4α itself constitutively binds fatty acids and is known to regulate lipid and glucose metabolism (Gonzalez, 2008), raising the prospect that this NR could influence DR in mammals.
Recently, the molting NR, NHR-23/ROR, has been tied to nutrient-dependent regulation of life history. Walhout and co-workers found that propagation of C. elegans on an alternative bacterial food source, Comomonas, results in developmental acceleration, reduced brood size, and shortened life span (MacNeil et al., 2013). Remarkably, this physiological response is observed even if trace amounts of Comomonas are mixed in with the normal food source, OP50, suggesting a nutrient sensing mechanism. However, these physiologic phenotypes appear independent of major nutrient sensing pathways such as TOR and IIS, and were instead associated with a dampening of gene expression of molt-cycle oscillatory as well as critical nutrient responsive genes such as acdh-1 (MacNeil et al., 2013). Transcriptome analysis further suggests that major expression changes could be mediated by nhr-23, although a definitive functional role for nhr-23 in regulating these life history traits remains to be seen (MacNeil et al., 2013). Additionally, the HNF-4 related NRs, nhr-10 and nhr-68, also mediate major changes in metabolic gene expression in response to Comomonas (Watson et al., 2013). These studies suggest that diet and metabolism are intimately linked with temporal regulation of development and that food quality as well as quantity can affect life history traits.
Interestingly, the molting NR, NHR-25/SF-1, also plays a role in fat metabolism. It was identified in a genetic screen for suppressors of the acyl-coA synthase gene acs-3 (Mullaney et al., 2010). Acyl-CoA synthases condense fatty acids of various lengths with acyl-CoA, and function in β−oxidation and other fat metabolic processes. acs-3 in particular has a preference for working on C18 fatty acids. Loss of function mutations in acs-3 result in increased fatty acid uptake, greater rates of de novo fatty acid synthesis, and accumulation of Nile red and bodipy-labeled fatty acids in large neutral lipid compartments in the C. elegans intestine (Mullaney et al., 2010). Surprisingly, acs-3 expression in epidermal seam cells triggers these metabolic effects in the gut, suggesting action at a distance. Suppressors of the acs-3 mutant include several other fatty acid metabolic genes, as well as hypomorphic mutations in nhr-25 (Mullaney et al., 2010). Conversely, acs-3 loss partially suppressed egg-laying and sterility phenotypes of nhr-25 hypomorphs, suggesting an antagonistic interaction. NHR-25 and its mammalian homologs SF-1 and LRH can accommodate fatty acyl-phosphoinositides in the LBD (Krylova et al., 2005; Urs et al., 2007; Mullaney et al., 2010; Lee et al., 2011). It is therefore surmised that acs-3 loss results in a depletion of long chain fatty acid-CoA inhibitory ligands for NHR-25, with the result that overactive NHR-25 provokes the observed changes in fat metabolism (Figure 10) (Mullaney et al., 2010). Because of mutual suppression, however, it is unclear whether nhr-25 acts upstream or downstream in these pathways. Curiously acs-3 mutants do not have a reported molting defect, presumably because NHR-25 still retains activity. Other genes implicated in fatty acid synthesis, however, do impact cuticle formation and expression of molting genes, namely pod-2, encoding acetyl-CoA carboxylase, and fasn-1, encoding fatty acid synthase (Li and Paik, 2011). Conceivably, fatty acid molecules could affect molting by modulating NR activity, or could provide mechanical integrity to the cuticle.
NHR-49 has emerged as one of the most important HNF4-like NRs, which coordinates fat metabolism in response to nutrient conditions, and whose responsibilities resemble that of mammalian lipid-sensing NRs, PPARα or PPARδ. Among other things, PPARα regulates expression of β−oxidation and fatty acid desaturase genes, and manages energy balance in response to starvation (Van Gilst et al., 2005a; Van Gilst et al., 2005b).
Consistent with a broad role in coordinating metabolism, NHR-49 resides in multiple tissues including intestine, hypodermis, body wall muscle, and pharynx (Van Gilst et al., 2005a). Among genes activated by nhr-49 in the fed state are those involved in mitochondrial β−oxidation of fatty acids (Table 2, Figure 10) (ech-1 enoyl-CoA-hydratase, acs-2 acyl-CoA synthase, cpt-5 carnitine palmitoyl transferase) the monodesaturation of fatty acids by Δ-9 fatty acid-CoA desaturases (fat-5, fat-6, fat-7), and lipid transport (lbp-8) (Van Gilst et al., 2005a; Van Gilst et al., 2005b). Additionally, nhr-49 promotes expression of genes in the glyoxylate cycle (gei-7, malate synthase; sdha-1, succinate dehydrogenase), which are involved in the conversion of fat to glucose. By contrast nhr-49 represses genes involved in peroxisomal β−oxidation (two acyl-CoA oxidases, ech-9) and lipid binding (lbp-7). It also inhibits expression of genes involved in sphingolipid processing, (acid ceramidase, glycosyl hydrolase, and sphingosine phosphate lyase) and lipid remodeling (a triacylglycerol lipase, an o-acyl transferase, two phospholipases) (Pathare et al., 2012). Ligands for nhr-49 remain unidentified but are hypothesized to be fatty acids or derivatives.
In support of the notion that nhr-49 coordinates fat usage, mutants exhibit lower levels of β-oxidation and oxygen consumption, and accumulate the vital dye Nile red, which stains a lysosomal-like acidic fat compartment (Pathare et al., 2012; Van Gilst et al., 2005a). As a result of a deficiency in Δ-9 fatty acid-CoA desaturase activity, they accumulate the saturated fatty acids C16:0 palmitic acid and C18:0 stearic acid (Van Gilst et al., 2005a). Accordingly, they have reduced C16:1n7 palmitoleic and C18:1n9 oleic monounsaturated fatty acids (MUFAs). Little change is seen in measured polyunsaturated fatty acid (PUFA) levels, although enzymes involved in their production (e.g., fat-1, fat-2) are also changed in quantitative proteomic experiments (Fredens et al., 2011).
The transcriptional response of nhr-49 very much depends on nutrient status. Notably, starvation induces a number of genes, some of which show nhr-49 dependence. These include acyl Co-A synthase genes, acs-2, acs-11, hacd-1, and gei-7 (Van Gilst et al., 2005b). Surprisingly, starvation also results in nhr-49(+) dependent suppression of cpt-4, revealing a complex regulation of genes involved in β-oxidation. Proteomic analysis confirms that nhr-49 plays a major role in fat metabolism, although its role in peroxisomal β-oxidation may be more important than that in mitochondrial β-oxidation, since many proteins reduced in nhr-49 mutants contain a peroxisomal localization signal (Fredens et al., 2011). nhr-49(+) may more broadly coordinate nutrient sensing systems since analyses of transcriptome, proteome, and metabolome data also reveals changes in amino acid, protein and xenobiotic metabolism (Atherton et al., 2008; Fredens et al., 2011; Pathare et al., 2012).
One of the most striking phenotypes of nhr-49 is its effect on life span. nhr-49 mutants are dramatically short lived compared to wild type (mean life span 9 days vs. 17 days) (Van Gilst et al., 2005a). Short life span is ascribed to lower expression of the fatty acid desaturases, and the higher ratios of saturated to unsaturated fatty acids, which can be lipotoxic in higher animals. In fact, several studies suggest an inverse correlation between the accumulation of saturated fat and longevity (Goudeau et al., 2011; Shmookler Reis et al., 2011), but the molecular basis of this correlation is unknown.
Although nhr-49 has no known role in dauer formation, it plays a critical role in a similar long-lived quiescent state termed the adult reproductive diapause (ARD), in which animals cease reproductive activities under starvation (Angelo and Van Gilst, 2009). Worms shifted to no food during the L4 stage stop embryo production and trim back germline stem cells through apoptosis or necrosis. A subset of animals retains arrested embryos for a week or so. Even after several weeks of starvation, animals sustain expression of the lag-2 Notch ligand, whose expression in the niche (gonadal distal tip cells) prevents meiotic progression and maintains the germline stem cells. When returned to ample food, animals will reconstitute their germline and other tissues, produce viable progeny, and live a normal life span, revealing protective mechanisms for soma and germline in response to nutrient deprivation, and robust rejuvenation in response to a return to food. Evidently nhr-49 is important for ARD, since fewer mutant animals enter the adult reproductive diapause (Angelo and Van Gilst, 2009). For those animals that successfully enter ARD, however, nhr-49 did not play an obvious role in the recovery of germline stem cells. Presumably nhr-49 promotes ARD by mobilizing metabolic genes important for energy homeostasis, stress, and turnover.
Interestingly, mammalian PPARα is also implicated in the response to fasting. PPARα transgenic mice massively upregulate expression of the fasting hormone FGF21, which turns on genes involved in hepatic β-oxidation, ketogenesis, and gluconeogenesis (Potthoff et al., 2012). Remarkably, FGF21 overexpression has been recently shown to be sufficient to improve insulin sensitivity and extend murine life span (Zhang et al., 2012). It does so by suppressing production of hepatic IGF-1, mimicking a state of growth hormone deficiency. Whether similar downstream pathways operate to regulate starvation induced longevity in C. elegans remains to be seen.
NHR-49 works in a number of transcriptional complexes to regulate metabolism. nhr-49 acts in conjunction with the co-activator mdt-15 to impact fatty acid metabolism (Figure 10). mdt-15 was found in yeast two-hybrid screens with nhr-49 as bait, and encodes a homolog of the mediator subunit 15, a multisubunit complex that functions as a transcriptional coregulator (Taubert et al., 2006). Consistent with the idea that mdt-15 and nhr-49 act together, both gene products are found in the intestine, the major metabolic and endocrine organ in the worm. mdt-15 loss of function results in a failure to accumulate nhr-49-dependent fasting induced genes, including gei-7, acs-2, acs-11, and hacd-1, as well as reduced expression of genes fat-5, fat-7, lbp-8, and cpt-5 in the fed state. mdt-15 mutants have perturbed fatty acid composition, increased saturated FA, reduced MUFAs and PUFAs, and are short lived. In fact, mdt-15 plays a broad role in the fatty acid/fasting response since it is required for regulation of additional genes, such as fat-2, acdh-1, acdh-2, ech-9, F08A8.2-4 acyl-CoA-oxidase and others, which are relatively unaffected by nhr-49. Accordingly mdt-15 physically interacts with a number of other NRs as well as the SREBP homolog sbp-1 (Yang et al., 2006; Arda et al., 2010). mdt-15 also has other nhr-49 independent roles including in PUFA production, as well as oxidative, xenobiotic and heavy metal metabolism (Taubert et al., 2008; Goh et al., 2013), and affects longevity in a number of contexts (Taubert et al., 2006; Schleit et al., 2011; McCormick et al., 2012).
In analogy to the mammalian heterodimeric partner RXR, NHR-49 also complexes directly with various other NRs (Figure 10). Namely nhr-49 interacts with nhr-66, nhr-80, nhr-13, nhr-22, and nhr-105, as measured through yeast two-hybrid interactions and/or GST-pulldowns (Pathare et al., 2012). Genetic and biochemical analysis reveals that these NRs execute separable aspects of nhr-49 function. For example, nhr-80 and nhr-13 specifically affect expression of fatty acid desaturase genes fat-5, fat-6, and fat-7. Similar to nhr-49, nhr-80 mutants accumulate saturated fats, have reduced monounsaturated fats, and are somewhat short lived. Although nhr-13 has no discernible change in fatty acid composition, mutants are also short lived. By contrast, nhr-66 functions specifically in the repression of genes involved in sphingolipid processing and lipid remodeling, and displays normal lifespan, suggesting that nhr-66 may carry out repressive aspects of nhr-49 function. nhr-49, nhr-80, and nhr-66 mutants have reduced β-oxidation, oxygen consumption, and altered mitochondrial morphology (Pathare et al., 2012). Altogether, these studies reveal that nhr-49 works in a combinatorial fashion in distinct complexes to carry out its diverse functions. It seems plausible that nhr-49 may interact with additional NRs.
Levels of saturated and desaturated fatty acids must be precisely controlled, and importantly, nhr-80 functions in homeostatic circuits that serve to regulate fatty acid desaturation gene expression and balance (Brock et al., 2006). As mentioned above, nhr-80 promotes expression of the Δ-9 fatty acid desaturases, particularly fat-7, fat-5, and to a lesser extent fat-6, under well-fed conditions (Figure 10). Correlatively, mutants accumulate C18:0 stearic acid and have reduced levels of C18:1n9 monounsaturated oleic acid (Brock et al., 2006; Pathare et al., 2012). Whereas the fat-7 fat-5; fat-6 triple mutant is lethal, single and double mutants are viable due to compensatory upregulation of the remaining intact genes by nhr-80. For example, when the fat-6 gene is mutated, the fat-5 and fat-7 genes are substantially upregulated. This upregulation depends largely on nhr-80(+): in the absence of the receptor, fat-7 induction is abrogated. As might be predicted, nhr-80 RNAi combined with fat-6 mutation exacerbates the imbalance in fatty acid composition. Moreover, nhr-80; fat-6 double mutants are synthetically lethal and only survive if provided with exogenous desaturated fatty acids (Brock et al., 2006).
NHR-80 is also required for longevity in response to signals from the reproductive system (Goudeau et al., 2011) (Figure 8). As mentioned above, C. elegans life span is extended considerably by removal of germline stem cells. Germline loss results in induction of nhr-80::gfp expression in the intestine, and deletion of nhr-80 suppresses the longevity of germlineless glp-1 mutants. One of the major transcriptional outputs of nhr-80 is the fat-6/Δ9 desaturase, which is strongly induced in germlineless animals in an nhr-80-dependent manner. Accordingly, germlineless animals exhibit elevated levels of C18:1n9 oleic acid, the metabolic product of FAT-6 activity. Consistent with an important physiological role, a double knockout of the fat-6 and fat-7 desaturases abrogates gonadal longevity, yet long life is restored by supplementation with oleic acid (Goudeau et al., 2011). These studies point to the importance of nhr-80 and fatty acid desaturation in extending life in response to germline signals. Germline removal also results in an upregulation of the lipase lipl-4 via DAF-16/FOXO (Wang et al., 2008) (Figure 8). lipl-4 overexpression is sufficient to extend life span in gonad-intact animals, while lipl-4 loss abrogates life span extension in germlineless glp-1 mutants, presumably due to FA released upon lipolysis. Interestingly lipl-4 overexpression has been shown to induce autophagy, and knockdown of autophagy gene function abrogates longevity (Lapierre et al., 2011). Omega-6 polyunsaturated fatty acids stimulate autophagy and longevity, revealing an important connection between fatty acid desaturation, autophagy, and longevity (O'Rourke et al., 2013). Conceivably oleic acid, omega-6 PUFAs, or their derivatives could stimulate longevity by altering fatty acid metabolism, directly stimulating autophagy, or working through nuclear receptor signal transduction.
Another HNF4-like receptor implicated in fat metabolism is NHR-64 (Liang et al., 2010). nhr-64 was identified in RNAi screens as a suppressor of the reduced Nile red staining and decreased fat storage phenotype of fat-6; fat-7 double mutants. Additionally, nhr-64 RNAi partially restores the low triacylglyceride levels, brood size and slow growth rates seen in fat-6; fat-7 mutants. As in mammals, the sterol regulatory element binding protein homolog, sbp-1 regulates Δ-9 desaturase expression, and levels of fatty acid desaturation (Yang et al., 2006). Like fat-6; fat-7 mutants, sbp-1 mutant animals fail to accumulate TAGs and exhibit slow growth phenotypes; nhr-64 RNAi also suppresses these phenotypes (Liang et al., 2010). An analysis of FA composition reveals that nhr-64 knockdown results in a reduction of saturated fatty acids, and an increase in monomethyl branched-chain fatty acids, which are important signals for cellular growth (Kniazeva et al., 2004; Zhu et al., 2013). In addition, several fatty acid metabolic genes show dysregulation upon nhr-64 knockdown. The pod-2 fatty acetyl-CoA carboxylase gene is substantially upregulated, revealing that fatty acid synthesis is increased. At the same time, the acyl-CoA-oxidase, F08A8.4, is decreased, suggesting that peroxisomal β-oxidation may be down (Figure 10). Accordingly, RNAi of F08A8.4 itself suppresses the slow growth phenotype of fat-6; fat-7 double mutants. Taken altogether, these results suggest that nhr-64(+) normally functions to remove fatty acids through oxidation and suppression of synthesis, and to thwart growth signaling. In this view, nhr-64 knockdown tempers fatty acid catabolism, and permits a more balanced metabolism in fat-6; fat-7 double mutants. Alternately, high levels of C18:0 stearic acid seen in fat-6; fat-7 mutants could overstimulate nhr-64 towards fatty acid catabolism and away from storage. In this view nhr-64 knockdown would decrease these activities. In the future it will be important to actually measure β-oxidation and other metabolic processes directly to interpret these observations.
The neurally produced biogenic amines, serotonin (5-HT) and octopamine, play an important role in regulating metabolism, feeding and behavior. In C. elegans they have been shown to synergistically induce fat loss in the intestine analogous to mammals where serotonin and beta-adrenergic signaling also cooperate (Noble et al., 2013). One of the prominent outputs of 5-HT treatment is the induction the major triacylglycerol lipase 1, atgl-1, expressed in the intestine. Interestingly, atgl-1 expression in this context is dependent on the NR NHR-76 (Figure 10) (Noble et al., 2013). Mutation of atgl-1 or nhr-76 prevents 5-HT-induced fat loss. Evidently this relay is indirect. Serotonin appears to first impinge upon the mod-1 serotonin-gated Cl-channel within URXR/L neurons, which are exposed to the body cavity and pseudocoelem. Presumably thereafter, unidentified molecules secreted from URX impinge on NHR-76 in the intestine, which promotes atgl-1 transcription, thus catalyzing FA lipolysis and breakdown. Interestingly, NHR-76 bears significant homology to RXR, which works as a heterodimeric partner with multiple nuclear receptors, and binds to retinoic acid derivatives. It will be especially interesting to see whether NHR-76 is ligand gated and whether it functions similar to RXR.
NHR-69 is one among the HNF4-like receptors that has been recently shown to function in glucose sensing, insulin signaling, dauer formation, and longevity (Park et al., 2012). NHR-69 protein was found associated in pulldown experiments with the R-smad homolog DAF-8, a component of TGF-β signaling that functions in dauer formation. Consistent with a role in dauer formation, mutation of nhr-69 mildly enhances the Daf-c phenotypes of mutants in TGF-β signaling. nhr-69 is expressed in the ASI, an important sensory neuron governing dauer formation and longevity. daf-8; nhr-69 double mutants synergize for a number of phenotypes indicative of reduced IIS, including increased life span and upregulated DAF-16/FOXO target gene expression. Most interestingly, double mutants exhibit decreased neuropeptide secretion, resulting from derepression of exp-2, an NHR-69 target gene encoding a conserved potassium channel (Park et al., 2012). This ion channel inhibits secretion of DAF-28, an insulin-like peptide expressed in ASI. Conversely, exp-2 loss of function results in insulin hypersecretion, and short life span. Genetic epistasis experiments suggest that exp-2 works downstream of nhr-69, but upstream of the daf-2/insulin receptor with respect to longevity. Additionally, nhr-69 expression in ASI is upregulated by IIS, presumably through positive feedback. Altogether these data suggest a model whereby an NHR-69-DAF-8 complex inhibits exp-2 expression, which results in insulin secretion and normal life (Figure 11). Upon downregulation of these transcription factors, exp-2 expression is elevated, thereby inhibiting insulin secretion, and leading to dauer formation and long life. Finally nhr-69 overexpression in the ASI neuron leads to a lethargic phenotype that is rescued by exogenous glucose. Thus NHR-69 behaves much like HNF4α in regulating glucose metabolism, insulin signaling, and metabolic homeostasis. Mammalian HNF4 and SMAD3 physically interact and evidence suggests conserved crosstalk between the pathways (Mizutani et al., 2011).
The HNF4α-like NR, NHR-31, has an extraordinarily specific role in tubulogenesis of the worm excretory cell (Hahn-Windgassen and Van Gilst, 2009), which functions analogously to the kidney tubule mediating ion and water balance in mammals. During larval development a single excretory cell in the head sends out paired tubular processes anteriorly and posteriorly which extend the length of the animal. nhr-31 is expressed within the excretory cell, as well as in the intestine and in unidentified tail cells. Deletion of the gene results in early embryonic lethality, presumably due to excretory cell dysfunction. RNAi knockdown of nhr-31 late in larval development results in sensitivity to high salt challenge (Hahn-Windgassen and Van Gilst, 2009). The excretory cell tubules in nhr-31 depleted animals harbor large varicosities, which contain a surplus of endoplasmic reticulum, mitochondria, and vacuole-like vesicles, as visualized by electron microscopy, which could be interpreted as regions of unregulated membrane growth or failure to turnover such organelles. Analysis of the transcriptional profile comparing nhr-31 RNAi to wild-type reveals a striking reduction in the expression of 16 subunits of the vacuolar ATPase, a proton pumping ATPase involved in lysosomal function and vesicle trafficking. Notably knockdown of these subunits induces a swelling of tubule varicosities similar to nhr-31 knockdown. The promoters of these V-ATPase genes are enriched in a putative binding site for NHR-31. Similarly, the mammalian HNF4α binding site is also enriched in the promoters of genes for the V-ATPase subunits, suggesting a possible related role for this receptor in kidney function.
Organisms in the wild encounter unpredictable food sources with differing levels of nutrients. The somatically expressed HNF4γ-like NR, NHR-114, affects the survival of germ cells in response to nutrient imbalance (Gracida and Eckmann, 2013). In nhr-114 deletion mutants, germ cells fail to proliferate normally, rendering the animal sterile. Curiously, this phenotype is observed on an E. coli B food source (e.g., OP50), but not with an E. coli K12 food source (e.g., HT115). This phenotype could in part be attributed to levels of dietary tryptophan, as supplementation of OP50 with tryptophan restored fertility. Moreover, rescue depended on live E. coli suggesting that tryptophan metabolites produced by the microbiome contribute to the response. An analysis of the NHR-114 transcriptome in response to tryptophan revealed changes in various xenobiotic and detoxifying genes. This suggests a role for HNF4γ in buffering tissue damage against dietary or metabolic insults, and a protective role of chemical defense in this response.
Several other less well-characterized NRs that affect various aspects of metabolism have emerged from genetic screens. Some have been identified through altered Nile red accumulation (Ashrafi et al., 2003), which marks a lysosomal-like organelle (O'Rourke et al., 2009). Others have been identified through stimulated Raman microscopy to visualize fat levels in vivo (Wang et al., 2011). The yeast one hybrid approach was used to systematically identify transcriptional regulators of defined sets of metabolic genes (Arda et al., 2010), and NRs emerged as an overrepresented transcription factor class, strongly implicating them in metabolic control. A network model of the interactions further revealed that multiple NRs converge on specific target genes, or regulate other NRs. Moreover, the MDT-15 co-activator was shown to interact with at least 12 such receptors. NHR-86 in particular emerged as a hub with 12 NRs converging on its promoter, and NHR-86 itself regulating several downstream NRs in this network. Consistent with a role in metabolism RNAi knockdown of these receptors resulted in alterations in Nile red accumulation, and some (e.g., nhr-178, nhr-45) showed nutrient dependent regulation, as well as predicted regulatory interactions in vivo (Arda et al., 2010). Clearly this powerful approach should be further exploited to expand the links in such networks, and used to elucidate the underlying physiology.
Studies in the worm demonstrate that NRs are key regulators of cellular fate, stage structure, metabolism, and longevity that can illuminate fundamental aspect of metazoan biology. Several challenges lie ahead for the future and many questions remains. What is the molecular architecture of the molt cycle and how does this integrate with sleep, metabolism, and developmental timing? What is the interrelationship between developmental timing components and longevity? How are the various NRs integrated into signal transduction pathways controlling cell fate and morphogenesis? What is the nature of metabolic networks and function of NRs in these circuits? What are the transcriptional regulatory networks in which NRs are embedded? What roles might NRs play in other physiologic functions, such as innate immunity, xenobiotic metabolism, behavior, and stress response? Beyond these physiologic questions are nuts and bolts issues of identifying the ligands, direct target genes, and coregulatory molecules that interface with the various NRs. Finally, what are the functional homologs of the diversified C. elegans NRs? The answers to these questions and many more are bound to yield important insights into evolutionarily conserved facets of metazoan NRs.
Table 1. C. elegans NRs
|Ce receptor||Expression||Function||Regulated Genes||Dm receptor||Function||Hs/Mm receptor||Function|
|DAF-12||neurons, intestine, hypodermis, vulva, pharynx, muscle, somatic gonad||dauer formation, developmental timing, fat metabolism, longevity regulated by dafachronic acid ligands||mir-241, mir-84, lit-1, lin-41, lin-42, lin-28, ain-1, nhl-2, din-1, cbp-1, daf-3, daf-16, fard-1, cdr-6||DHR96||cholesterol, TAG, xenobiotic metabolism, binds cholesterol||FXR/ NR1H3||cholesterol, fat, bile acid metabolism|
|NHR-8||intestine||dauer formation, fat metabolism, cholesterol metabolism, xenobiotic metabolism||daf-36, fat-5, fat-7, vit-1, vit-2, lipases, elo-1, fat-2||LXRα/ NR1H2, LXRβ/ NR1H1||glucose, cholesterol, fat, bile acid metabolism, neural development, immune function, skin barrier function and protection|
|PXR/ NR1I2, CAR/ NR1I3||xenobiotic and bile acid metabolism|
|VDR/NR11||bone development and homeostasis, phosphate and calcium homeostasis, cellular differentiation|
|UNC-55||motor-neurons||regulates remodeling of VD motoneuron synapses||represses hbl-1, Irx-1||Seven Up||neurogenesis, cardiac development||COUP-TF1/ NR2F1 COUP-TF2/ NR2F2||cardiovascular development, angiogenesis, neural development, pituitary and reproductive function, metabolism|
|NHR-25||intestine, hypodermis, vulva, pharyngeal and rectal epithelial cells, somatic gonad, germline||molting, epidermal differentiation, developmental timing, fat metabolism||apl-1, acn-1, mlt-10, nas-37||FTZ-F1||early embryonic patterning and larval molting and metamorphosis||SF-1/ NR5A1||regulates sexual development & development of steroidogenic tissues|
|LRH/ NR5A2||cholesterol, bile acid, glucose and fatty acid metabolism, inflammation, pluripotentency of embryonic stem cells, gut stem cell renewal|
|NHR-23||hypodermis, pharynx, germline, intestine?||molting, maturational timing in response to nutrients||dpy-2,3,5,7,8,10, wrt-1,2, 3, mlt-8,9,10, 11, ptc-3, acn-1, nhr-60||DHR3||ecdysone inducible, prepupal to pupal transition, metamorphosis, adult differentiation, molting, cellular growth||RORα/ NR1F1, RORβ/ NR1F2, RORγ/ NR1F3||circadian clock, cerebellum development, immunity, lipid, cholesterol, and glucose metabolism|
|NHR-41||seam cell, hyodermis, rectal epithelium, excretory cell, head neuron, excretory cell, vulva||Dauer molt||DHR78||ecdysone inducible, larval growth, metamorphosis, tracheal development||TR2/ NR2C1, TR4/ NR2C2||recruits corepressors, role in early embryonic development, and stem cells|
|NHR-85||excretory duct cell, hypodermis, rectal epithelium, vulva||dauer molt, egg laying,||E75||ecdysone inducible, covalently modified by heme and regulated by nitic oxide which regulates association with DHR3||REV-ERBα/ NR1D1, REV-ERBβ NR1D2||heme binding, circadian rhythm, adipogenesis|
|SEX-1||ubiquitous nuclear staining in embryo||sex determination||xol-1||E78||Ecdysone inducible, Repressed by DHR3||“||“|
|NHR-67||head neurons, hypodermis, vulva, somatic gonad, excretory cell||epidermal morphogenesis, vulval and gonadal development, neuronal cell fate, excretory canal morphogenesis||egl-17/FGF, egl-26/ LRAT, pax-2, zmp-1, unc-5 (LC), hlh-8, crh-2, sphk-1, trpa-1, che-1||Tailless||inhibits embryonic segmentation, promotes terminal fates, neurogenesis||TLX/ NR2E1, TLL/ NR2E2||nervous system and visual development, embryonic and adult neural stem maintenance|
|FAX-1||AVK, various interneurons||interneuron fate specification, axon outgrowth, neurotransmitter expression||flp-1, opt-3, ncs-1||DHR51||mushroom body development, wing expansion fertility||PNR/ NR2E3||photoreceptor rods and cones|
|NHR-6||ASI, spermatheca||spermathecal organogenesis||cog-1, let-502||DHR38||molting and metamorphosis, glycogen storage, carbohydrate metabolism||Nurr77/NR4A1, Nurr1/NR4A2, NOR1/NR4A3||immediate early response, neural development, specification of midbrain dopaminergic neurons, immunity and inflammation, atherogenesis, adrenal steroidogenesis, and glucose and mitochondrial metabolism|
|NHR-91||neurons, seam cells, vulva, excretory cell, spermatheca,||regulates stem blast cell quiescence and progression in response to nutrients||DHR4||controls the timing of ecdysone pulses and larval maturation, through negative feedback||GCNF/NR6A1||transcriptional repressor required for vertebrate stem cell differentiation, embryogenesis and neural differentiation|
Table 2. Spotlight on functionally characterized HNF4-related receptors.
|Receptor||Tissue Expression||Function||Regulated genes|
|NHR-62||neurons, intestine, hypodermis, pharynx, spermatheca, excretory cell||Promotes: dietary restriction mediated longevity||acdh-2, lbp-8, vit-1-6 col-91, gst-21, lgg-1|
|NHR-49||neurons, intestine, hypodermis, pharynx, body muscle||Promotes: FA β oxidation, FA desaturation, transport, adult reproductive diapause||fat-5, fat-6, fat-7, lbp-8 cpt-5, ech-1, acs-2, acs-11, hacd-1, gei-7, acid ceramidase F27e5.1, glycosylhydrolase E02H9.5, spl B0222.4, ZK167.2 lipase, W02b12.1 lipase|
|NHR-80||neurons, intestine, head muscle||Promotes: FA desaturation, β–oxidation, gonadal longevity||fat-5, fat-6, fat-7, cpt-5, ech-1|
|NHR-66||neurons in anterior, lateral, retrovesicular ganglia, seam cells, hypodermis, intestine, body muscle||β oxidation, Represses: sphingolipid metabolism||ceramidase F27e5.1, glycosylhydrolase E02H9.5, spl B0222.4, ZK167.2 lipase, W02b12.1 lipase|
|NHR-13||ubiquitous, intestine||Promotes: FA desaturation||fat-5, fat-6, fat-7, ech-1|
|NHR-76||body muscle, vulval muscle, excretory gland, intestine, seam cell||Promotes: FA lipolysis in response to serotonin||atgl-1|
|NHR-64||neurons of head, tail, ventral cord, intestine, hypodermis, pharynx||Promotes: FA synthesis Prevents: FA catabolism||acs-1-5, elo-5, elo-6, pod-2 up, acyl-CoA-oxidase F08A8.4 down|
|NHR-69||ASI neuron, tail neurons, Intestine, hypodermis, pharynx, uterus, rectal epithelium||Glucose metabolism, TGF-β & Insulin signaling, Dauer formation, longevity||exp-2|
|NHR-31||excretory cell||Excretory cell morphogenesis, Fluid Balance||Vacuolar-ATPase subunits|
|NHR-114||intestine, hypodermis, germline||Germ Cell Maintenance, Xenobiotic Metabolism||cyp-35b2, cyp-35B1, clec-61, clec-169, gst-8, acs-10, ugt-63|
|NHR-40||neurons, body muscle, pharynx, vulval muscle, rectal glands, P ectoblasts||Embryonic muscle development||myo-2, act-1, unc-52|
|NHR-60||VC4 -, VC5 -motor-neurons, pharyngeal gland, seam cell, male ray UV1 uterine cells||Ventral closure, embryogenesis|
I thank Dan Magner and Philipp Jäger for critical reading of the manuscript and Moritz Horn, Christoph Geisen, and Sabine Finkler for help with figures. I thank the Max Planck Society, Deutsche Forschungsgemeinschaft (SFB635 & Cluster of Excellence: Cellular Stress Responses in Aging-Associated Diseases (CECAD)), and the German Bundesministerium für Bildung und Forschung (Gerontosys; Sybacol) for their generous support.
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*Edited by Iva Greenwald. Last revised July 23, 2014. Published June 9, 2015. This chapter should be cited as: Antebi A. Nuclear receptor signal transduction in C. elegans (June 9, 2015), WormBook, ed. The C. elegans Research Community, WormBook, doi/10.1895/wormbook.1.64.2, http://www.wormbook.org.
Copyright: © 2015 Adam Antebi. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
§To whom correspondence should be addressed. E-mail: AAntebi@age.mpg.de
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