From The Archive: ()

Cover artwork: C. elegans The New Generation
Greg Nelson
Cover Artwork: A visit to Nara: caution, deer might bite or butt
Greg Nelson
Front Cover: The Transcribe
Greg Nelson
Cover Artwork: I C.e. you
Greg Nelson
Cover Artwork
Slitherin’ and Slytherin
Greg Nelson
Cover Artwork: Alice in Wormderland
Greg Nelson
Cover Artwork: Wild types
Greg Nelson
Cover artwork: Elegant Genesis
Beverley Dancy and Andrea Choe
Cover Artwork: Casablanca
Greg Nelson
Editorial: The Return of The Worm Breeder’s Gazette
Martin Chalfie, Editor-in-Chief, WormBook
Antibiotic markers for rapid selection and easy maintenance of transgenic nematodes
Rosina Giordano-Santini, Jennifer Semple, Denis Dupuy and Ben Lehner
A rapid nematode preparation for microscopy
Shamol Saha, Vivek Gowda, Chelsea Trengrove and Benjamin Wolozin
Introducing: New types of Gazette content
Jane Mendel, Editor, WormBook
Worm humor: Nematode news in brief
Decrepitus W. Picker
Nematode news in brief
Decrepitus W. Picker
Call for feedback for possible rearrangements in deletion mutants
Shohei Mitani
WormBase private instances via Amazon’s Cloud infrastructure
Todd Harris and Lincoln Stein
Rapid generation of deletion alleles using CRISPR/Cas9
Selma Waaijers and Mike Boxem
CTGC – a portal for distributing transgenic worms
Gunasekaran Singaravelu
Counting endogenous mRNA molecules in C. elegans
Dong hyun Kim and Alexander Oudenaarden
The wet plate protocol: an efficient way to obtain dauer larvae
Andreas M. Weller
The million mutation project – a genetic resource for C. elegans.
Donald G. Moerman, Pnina Strasbourger, Owen Thompson, Ryan Adair, Vinci Au, Iasha Chaudhry, Mark Edgley, Brent Ewing, Lisa Fernando, Stephane Flibotte, Amber High, LaDeana Hillier, Harald Hutter, Joanne Lau, Norris Lee, Angela Miller, Greta Raymant, Jay Shendure, Jon Taylor, Emily H. Turner and Robert Waterston
Keeping up with the changes
Aurelien Griffon and Jonathan Ewbank
WormBox: a parallelized webcam recording system for nematode bioassays
Gregory S. Stupp and Arthur S. Edison
Pre-approval of strains donated to the CGC
Aric Daul and Ann Rougvie
A fast, marker-free method to mutate, tag, or delete any gene using linear DNAs with short homology arms
Alexandre Paix and Geraldine Seydoux
Meeting announcement: C. elegans Development, Cell Biology & Gene Expression
Ahna Skop
Reverse chunking, a simple and effective method for identifying unc-119(+) transgenics
Kevin F. O'Connell
Cloning eat genes using whole-genome sequencing
Mooncheol Park and Leon Avery
An update on mutant identification by combined SNP mapping/whole genome sequencing and CloudMap data analysis
Gregory Minevich, Feifan Zhang, Ines Carrera, Nikos Stefanakis, Marie Gendrel, Sze Yen Kerk, Paschalis Kratsios, Michael Hart, Abhishek Bhattacharya and Oliver Hobert
Cloning of eat-17
Sarah Straud and Leon Avery
glp-1(e2141) sequence correction
Diana Dalfó, James R. Priess, Ralf Schnabel and E. Jane Albert Hubbard
Visualizing the spatial distribution of biomolecules in C. elegans by imaging mass spectrometry
Yoshishige Kimura, Yuki Sugiura, Takahiro Hayasaka and Mitsutoshi Setou
Inhibiting C. elegans movement with ethanol for live microscopy imaging
Alicia E. Woock and Jennifer Perry Cecile, Dr.
WormWiring: A new online resource for nematode connectivity
Steven Cook, Christopher Brittin, David Hall and Scott Emmons
Endogenous locus modification by genome engineering: proposed nomenclature
Tim Schedl and Jonathan Hodgkin
HOT operons!
Gary Williams
From injection to mutant in four days: a hybrid PCR-phenotypic CRISPR-Cas screen
Kristen L. Fowler, Leanna R. Rucker, Paige M. Estave and Peter L. Barrett
Strains for neuronal RNAi and mec-8-dependent splicing
Andrea Calixto, Irini Topalidou, Xiaoyin Chen, Dattananda S. Chelur, Charles Ma and Martin Chalfie
Modifying the gene model of rrf-3
Ayelet T. Lamm, Jonathan I. Gent and Andrew Z. Fire
Triton X decreases adherence of C. elegans to pipette tips in liquid medium
Gregory M. Davis, Madhu Sharma, Lloyd Low and Peter R. Boag
MiModD – mutation identification from whole-genome sequencing data on desktop PCs
Katharina Moos, Mark Seifert, Ralf Baumeister and Wolfgang Maier
Transcription factor zfp-1 enhances cell death defects in C. elegans
Robert Pollok
Worms get a brand new glue
Shana L. Geffeney, Miriam B. Goodman and the Worm Neurobiologist Community
NucleiTracker4D: A toolbox for semi-automated analysis of embryonic lineages and morphogenetic movements
Claudiu A. Giurumescu, Alan Suk-Ryool Kang, Pamela Cosman and Andrew D. Chisholm
An online OrthoList tool to query worm-human gene homology
Daniel Shaye, Woojin Kim, James Chen and Iva Greenwald
A simple, quantitative immunoblot protocol using equal numbers of nematodes
Shamol Saha and Benjamin Wolozin
CRISPER’ed twitchers
Wadim Kapulkin and Mihail Sarov
Whole genome sequence analysis for novices
Pete Okkema and Adelaide Packard
A simple yet effective method to harvest worms from a liquid culture
Teruaki Takasaki, Toshinobu Fujiwara and Susan Strome
CRISPR/Cas C. elegans web sites
Jane Mendel
UCSC Genome Browser
Raymond Lee
An altered method of feeding RNAi for multiple genes in C. elegans
Kenji Gouda, Yohei Matsunaga, Yukari Omichi, Takashi Iwasaki and Tsuyoshi Kawano
Protein extraction from C. elegans using sea sand
Shamol Saha, Julien Dusonchet and Benjamin Wolozin
Using LEDs as a low-cost source to detect GFP and DsRED
Ian Chin-Sang and Weiwei Zhong
Rapidly identifying spontaneous mutants via next generation sequencing
Freya Shephard, Jeff Chu, David L. Baillie, Ann Rose and Nathaniel J. Szewczyk
snPCR for reliable one-step genotyping of single nucleotide differences
Anca Neagu and Wolfgang Maier
Suppressors of eat-5, a new type of peptide hormone?
Leon Avery and Brian Chim
JFlux – a platform for flow cytometry with focus on the COPAS biosorter
Johan Henriksson and Thomas R. Bürglin
Few gene expression differences between C. elegans grown in liquid versus on plates
Valerie Reinke, Frederick G. Mann, Christina M. Whittle and Jason D. Lieb
A multiply fluorescent strain for outcrossing Million Mutation Project alleles and linkage mapping
Jonathan Hodgkin
C. elegans CRISPR/Cas papers
Jane Mendel
par-1 allele update: b274 is a premature stop and encodes a delocalized but active kinase
Erik Griffin, Adrian Cuenca and Geraldine Seydoux
Mapping the breakpoints of the daf-16(mgDf50) allele
Annabel Ferguson and Alfred Fisher
Using magnetism to move C. elegans
Peter Wawrzusin, Yicong Wu, Peter Winter and Hari Shroff
A library of expression clones and cellular manipulation tools
Brigitte LeBoeuf and Rene Garcia
Analysis of high complexity fosmid-based transgenes in C. elegans
Luisa Cochella, Stephane Flibotte, Jon Taylor, Nikolaos Stefanakis, Gregory Minevich, Donald Moerman and Oliver Hobert
Omission of RNase and addition of ethanol precipitation improve transgenesis using spin column-purified DNA
Morris Maduro
A universal Cbr-unc-119::FRT*::galk::FRT* template for recombineering and biolistics
Patricia E Kuwabara and Mengmeng Qiao
A protocol for constructing and characterizing transgenic nematodes expressing stress-tolerant gene TPS1 of yeast in dauer larva stage
Zsófia Csanádi, Katalin Bélafi – Bakó1, Abate Birhan Addisie, Anita Alexa, Barnabás Jenes, Zsófia Bánfalvi, Éva Lehoczky and András Fodor
Live worm respiration in 24-well format
Beverley M. Dancy, Ernst-Bernhard Kayser, Margaret M. Sedensky and Philip G. Morgan
Media osmolarity modulates dopamine-dependent, swimming-induced paralysis (SWIP)
J. Andrew Hardaway, Sarah M. Whitaker and Randy D. Blakely
The Peptide-GPCR Project: matching C. elegans neuropeptides to their cognate receptors
Isabel Beets, Katleen Peymen, Liesbet Temmerman and Liliane Schoofs
Robust expression of transgenes in the C. elegans germline through a simple microinjection protocol.
Luisa Cochella, Baris Tursun, Nikolaos Stefanakis, Dylan Rahe, Inés Carrera and Oliver Hobert
Binding Partners for UNC-89 (Obscurin): How to Catch Moby Dick
Hiroshi Qadota, Kristy J. Wilson, M. Berenice Duran, Ge Xiong and Guy M. Benian
The expression profile of innexins in the C. elegans male
María I. Lázaro-Peña and Scott W. Emmons
Temperature sensitive mutants of the RNA polymerase II TFIID initiation factor, taf-6.2
Elizabeth Bowman, Geraldine Seydoux and William Kelly
Sharing is caring, helping is developing
Callista Yee and Steve Hodgkinson
A better worm pick handle
Paul Sternberg
A couple of new tools for the disorganized or pedantic researcher
Jonathan Ewbank
Unnatural amino-acid tagging and read-through of nonsense stop codons in multicopy transgene arrays
Emily Barker, Maria Joao Gravato-Nobre and Jonathan Hodgkin
Back Cover: Worms on Ice
Malina Bakowski
Quantifying roaming behavior in synaptic defective mutants of C. elegans
Fernando Calahorro and Manuel Ruiz-Rubio
Using the CRISPR/Cas9 system to target cis-regulatory sequences in C. elegans
Michalis Barkoulas, Aurelien Richaud and Marie-Anne Félix
A modified leaving assay to study pathogen avoidance
Alexandra Anderson and Rachel McMullan
A C. elegans distal tip cell-specific RNAi screen
R. Daniel Slone, Dana Byrd, Judith Kimble and Jean E. Schwarzbauer
Isolation of C. elegans 80S ribosomes
Cheng-Yang Wu and Michael Roth
Identification of a binding partner for UNC-89 (obscurin) as a new focal adhesion protein, CPNA-1, required for muscle development in C. elegans
Ge Xiong, Adam D. Warner, Hiroshi Qadota, Teresa Rogalski, Donald G. Moerman and Guy M. Benian
Using feeding RNAi to enhance visualization of neuronal gfp expression
Xiaoyin Chen and Martin Chalfie
Artifacts in L1 starvation assay
Alexander B. Artyukhin and Leon Avery
Towards an automatic method for toxicity and pharmacological testing in C. elegans
Sergio H. Simonetta
Visualization of embryonic development with a downloadable 4D quantitative model
Amanda L. Zacharias, Travis Walton, Joshua T. Burdick and John I. Murray
High dose of D-glucose enhances cell growth in primary cultures of C. elegans cells
Koji Yamada and Yukio Nakamura
A simple method for quantifying M. nematophilum clearance from the rectal opening of C. elegans
Alexandra Anderson, Shinon Kang and Rachel McMullan
Making linear chemical gradients in agar
Linjiao Luo, Joel Greenwood, Edward Soucy, Daniel Kim and Aravinthan Samuel
The “sneeze” response: worms avoid piperine, the active component of black pepper
Dung T. Duong, Rebekah R. Sadaiappen and Peter L. Barrett
Batch_Fusion_Primer: a tool to generate the necessary primers for GFP fusion experiments from files containing any number of sequences
Damien O'Halloran
Prevalent, spontaneously occurring mutations at the eri-6/7 locus result in an Eri phenotype
Sylvia E. J. Fischer, Chi Zhang and Gary Ruvkun
How to measure lethargus quiescence without monopolizing the dissection microscopes in your lab
Huiyan Huang, Chen-Tseh Zhu and Anne Hart
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