WormMethods - Oliver Hobert, ed.

Basic methods for C. elegans

• Maintenance of C. elegans (2006) - Theresa Stiernagle html pdf
• Nomarski images for learning the anatomy, with tips for mosaic analysis (2006) - John Yochem html pdf
• A biologist's guide to statistical thinking and analysis (2013) - David S. Fay and Ken Gerow html pdf
• Web resources for C. elegans studies (2005) - Raymond Lee, ed. html pdf
• An introduction to the UCSC Genome Browser (2013) - Raymond Lee html pdf
•   An Introduction to Worm Lab: from Culturing Worms to Mutagenesis (2011) - Jyotiska Chaudhur, Manish Parihar and Andre Pires-daSilva - JoVE video
•   Basic Caenorhabditis elegans Methods: Synchronization and Observation (2012) - Montserrat Porta-de-la-Riva, Laura Fontrodona, Alberto Villanueva and Julián Cerón- JoVE video

Forward and reverse genetics

• Classical genetic methods (2013) - David S. Fay html pdf
• Forward and reverse mutagenesis in C. elegans (2014) - Lena M. Kutscher and Shai Shaham html pdf
• Genetic balancers (2006) - Mark L. Edgley, David L. Baillie, Donald L. Riddle, and Ann M. Rose html pdf
• Genetic dissection of developmental pathways (2006) - Linda S. Huang and Paul W. Sternberg html pdf
• Reverse genetics (2006) - Julie Ahringer, ed. html pdf
•   Transgenesis in C. elegans (2011) - Vida Praitis and Morris F. Maduro
•   Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans (2012) - Barbara Squiban, Jérome Belougne, Jonathan Ewbank and Olivier Zugasti - JoVE video
•   RNAi Screening to Identify Postembryonic Phenotypes in C. elegans (2012) - Katherine K. Beifuss and Tina L. Gumienny - JoVE video
•   Specialized chromosomes and their uses in C. elegans (2011) - Martin R. Jones, Aoe Lohn and Ann M. Rose

Microinjection and transformation

• C. elegans gene transformation by microparticle bombardment (2013) - Peter J. Schweinsberg and Barth D. Grant html pdf
• Transformation and microinjection (2006) - Thomas C. Evans, ed. html pdf
• Transgenic solutions for the germline (2010) - Christopher Merritt, Christopher M. Gallo, Dominique Rasoloson and Geraldine Seydoux html pdf
•   Generation of Stable Transgenic C. elegans Using Microinjection (2008) - Laura A. Berkowitz, Adam L. Knight, Guy A. Caldwell and Kim A. Caldwell - JoVE video
•   Generation of Transgenic C. elegans by Biolistic Transformation (2010) - Daniel Hochbaum, Annabel A. Ferguson and Alfred L. Fisher - JoVE video

Measuring and monitoring gene expression

• Immunohistochemistry (2006) - Janet S. Duerr html pdf
• RNA in situ hybridization of dissected gonads (2006) - Min-Ho Lee and Tim Schedl html pdf
• In situ hybridization on whole mount embryos of C. elegans (2005/2015) - Tomoko Motohashi, Keiko Hirono, and Yuji Kohara html pdf
• Protocols for large scale in situ hybridization on C. elegans larvae (2006) - Tomoko Motohashi, Hiroaki Tabara and Yuji Kohara html pdf
• Reporter gene fusions (2006) - Thomas Boulin, John F. Etchberger, Oliver Hobert html pdf
• DNA microarrays (2006) - Douglas S. Portman html pdf
• Single molecule fluorescent in situ hybridization (smFISH) of C. elegans worms and embryos (2012) - Ni Ji and Alexander van Oudenaarden html pdf
•   Immunofluorescence microscopy (2012) - Diane C. Shakes, David M. Miller III, and Michael L. Nonet
•   Fluorescent protein methods (2012) - Harald Hutter
•   In situ hybridization of embryos with antisense RNA probes (2011) - Gina Broitman-Maduro and Morris F. Maduro

Microscopy and other instrumentation

• Keeping track of worm trackers (2012) - Steven Husson, Wagner Steuer Costa, Cornelia Schmitt, and Alexander Gottschalk html pdf
• Microfluidics as a tool for C. elegans research (2013) - Adriana San-Miguel and Hang Lu html pdf
•   Cell identification and cell lineage analysis (2011) - Claudiu A. Giurumescu and Andrew D. Chisholm
•   Imaging embryonic morphogenesis (2011) - Jeff Hardin
•   Modern electron microscopy methods for C. elegans (2012) - David H. Hall, Erika Hartwieg and Ken C.Q. Nguyen

Cell and molecular biology

• Biochemistry and molecular biology (2006) - Albertha. J. M. Walhout and Simon J. Boulton html pdf
• Methods in cell biology (2006) - Shai Shaham, ed. html pdf
• Cell isolation and culture (2013) - Sihui Zhang and Jeffrey R. Kuhn html pdf
• Mass spectrometry-based shotgun proteomic analysis of C. elegans protein complexes (2014) - Bryan R. Fonslow, James J. Moresco, Patricia G. Tu, Antti P. Aalto, Amy E. Pasquinelli, Andrew G. Dillin and John R. Yates III html pdf
• Modern techniques for the analysis of chromatin and nuclear organization in C. elegans (2014) - Peter Askjaer, Sevinç Ercan and Peter Meister html pdf
•   Laser microsurgery in Caenorhabditis elegans (2012) - Christopher Fang-Yen, Christopher V. Gabel, Aravinthan D.T. Samuel, Cornelia I. Bargmann and Leon Avery
•   Analysis of membrane-bound organelles (2012) - Hanna Fares and Alexander M. van der Bliek
•   Culture and Manipulation of Embryonic Cells (2012) - Lois G. Edgar and Bob Goldstein

Behavioral assays

• Behavior (2006) - Anne Hart, ed. html pdf
• Assaying mechanosensation (2014) - Martin Chalfie, Anne C. Hart, Catharine H. Rankin, and Miriam B. Goodman html pdf
• Methods for measuring pharyngeal behaviors (2012) - David Raizen, Bo-mi Song, Nick Trojanowski, and Young-Jai You html pdf
• Thermotaxis navigation behavior (2014) - Miriam B. Goodman, Mason Klein, Samuel Lasse, Linjiao Luo, Ikue Mori, Aravi Samuel, Piali Sengupta, and Dong Wang html pdf
• Working with dauer larvae (2018) - Xantha Karp html pdf
•   Analysis of aging in Caenorhabditis elegans (2012) - Deepti S. Wilkinson, Rebecca C. Taylor and Andrew Dillin

Neurophysiological methods

• Neurophysiological methods in C. elegans: an introduction (2006) - William R. Schafer html pdf
• Culture of embryonic C. elegans cells for electrophysiological and pharmacological analyses (2006) - Laura Bianchi and Monica Driscoll html pdf
• Electrophysiological recordings from the neuromuscular junction of C. elegans (2006) - Janet E. Richmond html pdf
• Electrophysiological recordings from the pharynx (2006) - Alan Cook, Christopher J. Franks and Lindy Holden-Dye html pdf
• Heterologous expression of C. elegans ion channels in Xenopus oocytes (2006) - Laura Bianchi and Monica Driscoll html pdf
• Imaging the activity of neurons and muscles (2006) - Rex A. Kerr html pdf
•   Optogenetic manipulation of neural activity in C. elegans: From synapse to circuits and behaviour (2013) - Steven J. Husson, Alexander Gottschalk and Andrew M. Leifer

Methods for nematode species other than C. elegans

• Caenorhabditis briggsae methods (2006) - Scott Baird and Helen Chamberlin html pdf
• Isolation of C. elegans and related nematodes (2014) - Antoine Barrière and Marie-Anne Félix html pdf
• Pristionchus pacificus protocols (2013) - Andre Pires da Silva html pdf
• Pristionchus pacificus genomics: from genetics to genome sequence (2006) - Christoph Dieterich, Waltraud Roeseler and Jagan Srinivasan html pdf
• Strongyloides stercoralis: a model for translational research on parasitic nematode biology (2007) - James B. Lok html pdf
• Transgenesis in animal parasitic nematodes: Strongyloides spp. and Brugia spp. (2013) - James B. Lok and Thomas R. Unnasch html pdf

Discuss C. elegans WormMethods on the WormBase forum